GSTM1基因多态现象与原发性肝癌遗传易感性的关系研究

Relationship Between GSTM1 Gene Polymorphism and Genetic Susceptibility to Primary Hepatocellular Carcinoma

目的 探讨谷胱甘肽S转移酶M 1(GSTM 1)基因多态现象与原发性肝癌遗传易感性的关系。方法 应用多重PCR方法检测 5 4例肝癌患者 (病例组 )和 136例健康人 (对照组 )的GSTM 1空白基因型。结果 病例组GSTM 1空白基因型频率为70 .37% ,对照组则为 45 .5 9% ,两者有非常显著性差异 (P <0 .0 1) ,但 2组均不存在性别、年龄差异 (P均 >0 .0 5 ) ;OR值为 2 .83(95 %CI值为 1.43～ 5 .42 ) ,EF值为 0 .5 3 ,具有GSTM 1空白基因型的个体患肝癌危险性增加 1.83倍。结论 GSTM 1基因多态现象与肝癌遗传易感性有密切的关系 ,提示其空白基因型可作为判断肝癌遗传易感性的指标。

Objective To study the relatioship between glutathione S-transferase M1(GSTM1) gene polymorphism and genetic susceptibility to primary hepatocellular carcinoma(PHC).Methods The GSTM1 genetypes of 54 patients with PHC and 136 healthy controls were detected by multiple PCR technique.Results The frequency of GSTM1 null genotype was 70.37% in the patients,and 45.59% in controls,the difference was statistically significant( P <0.01).The odds ratio was 2.83(95%CI=1.43～ 5.42 ),and the etiologic fraction was 0.53,which suggested that the persons with GSTM1 null genotype has 1.83 times of increased risk of PHC,and 53% of PHC cases in the population can be attributable to the GSTM1 null genotype.Conclusion There is a remarkable relationship between GSTM1 genetic polymorphism and genetic susceptibility to PHC,which is determined by GSTM1 null genotype.