肺癌p53蛋白和增殖细胞核抗原的检测及其意义

Expression and Significance of p53 Protein and PCNA in Primary Lung Cancer

目的 探讨p5 3蛋白、PCNA联合表达在临床中的意义。方法 应用免疫组织化学S P法检测 10 1例肺癌组织中p5 3蛋白、PCNA的表达。 结果 p5 3蛋白在肺癌组织中有较高的表达 ,总阳性率为 5 9.4% (6 0 /10 1)。p5 3蛋白表达与肿瘤分化程度呈正相关 (P <0 .0 5 ) ,而与肺癌患者的生存期呈负相关 (P <0 .0 1)。p5 3蛋白表达与PCNA染色密切相关 (P <0 .0 5 )。 结论 p5 3蛋白表达可作为判断肺癌患者预后的 1个重要参考指标。

Objective To investigate the relationship between p53 protein and proliferating cell nuclear antigen(PCNA) and the clinical significance in primary lung cancer.Methods S-P immunohistochemistry method was used in 101 cases of primary lung cancer tissue.Results Abnomalities in p53 expression were found,the positive ratios was 59.4%(60/101).p53 overexpression were associated with tumor grading and survival time( P <0.05).A significantly higher PCNA expression score was found in the p53-positive samples( P <0.05).Conclusion This finding suggests that overexpression of p53 could be used as an independent prognostic factor for the patients with lung cancer.