血清p53抗体水平与肺癌临床指标关系的研究

Relationship Between Serum Anti-p53 Antibodies and Clinical Characteristics of Lung Cancer

目的 通过对肺癌患者血清p5 3抗体水平与肺癌临床指标关系的研究 ,探讨血清 p5 3抗体在肺癌中的临床意义。 方法 对 6 8例肺癌患者应用ELISA法检测血清 p5 3抗体滴度 ,经酶联免疫检测仪测吸光值E4 50 ,计算抗体指数 ,进行统计学分析。结果 6 8例中阳性 32例 ,阳性率为 47.1%。血清 p5 3抗体水平与肺癌的一般临床特征 ,如性别、分期、吸烟指数、既往治疗均无关 ,而与肺癌的病理类型和肿块大小有关。结论 p5 3抗体的产生可能是肺癌发生的早期指征 ,是肺癌的不良预后因子 ,有望应用于预测复发和评价疗效。

Objective To detemine the relationship between serum anti-p53 antibody level and clinical characteristics of lung cancer.Methods In 68 lung cancer patients,anti-p53 antibodies were determined with ELISA and the absorbance(E 450 ) was measuresed with a microplate reader.Then the anti-p53 index was calculated and statistical analyses were performed.Results 32 positive cases were observed in the study and the positive rate is 47.1%.No significant differences were found between anti-p53 index and sex,smoking index,disease stage and previous treatment.But anti-p53 level was related with the histological type and the size of tumour.Conclution It is shown that the production of anti-p53 antibody is an early event.The serum anti-p53 antibody is a prognostic factor and might be used in the relapsing prediction and the therapeutic evaluation of lung carcinoma.