当归补血汤治疗脑缺血的实验研究

Study on the Curative Effect on Cerebral Ischemia in Gerbils with the Danggui Complex Mixture

为观察当归补血汤对脑缺血的疗效 ,使用穿梭箱连续观察用当归补血汤组 (n =2 4 )和未给药组 (n =2 4 )脑缺血模型沙土鼠学习记忆的改变。并取海马区组织块 ,光镜下观察海马区组织形态的变化。结果 :模型组沙土鼠记忆功能比术前显著降低 (P <0 .0 1 ) ,给药组比未给药组动物的记忆功能恢复得快 (P <0 .0 1 ) ;模型组海马区组织镜下显示病理性损伤 ,锥体细胞数目比正常组明显减少 (P <0 .0 1 ) ,给药组的锥体细胞数目明显多于未给药组 (P <0 .0 1 )。结果提示当归补血汤能够减轻脑缺血后的病理损伤 ,能够明显改善脑血液循环 ,促进脑生理机能的恢复 ,对脑组织具有一定保护作用。

In order to observe the curative effect on cerebral ischemia in the gerbils with the Danggui (Angelica sinensis) complex mixture, the change of the study and memory ability of the gerbils in the cerebral ischemic model group was observed by means of the shuttle box. Morphological change in their hippocampal tissues was observed under the microscopes. The sections of the hippocampal tissue were made use of routine HE, Nills staining. After ligating their unilateral carotid, the memory ability of the gerbils in the model group was significantly lower than that in the preopertive normal value ( P <0.01). The regain of memory ability of the gerbils in the treated group with the traditional Chinese medicine ( n = 24) was significantly faster than that in the control group ( n = 24) ( P <0.01). The hippocampal tissue in the model group showed the pathological harm. The pyramid cells of hippocampus in the model group were less than that in the normal group ( P <0.01), and the pyramid cells in the treated group were more than those in the control group ( P <0.01). The result showed that the Danggui complex mixture could reduce the cerebral pathological harm,promote to regain the physiologic function of postischemic brain,improve the cerebral circulation, and protect on the brain.