日本血吸虫病流行区人群吡喹酮治疗前后细胞因子水平的研究

STUDIES ON HUMAN CYTOKINE RESPONSES BEFORE AND AFTER PRAZIQUANTEL CHEMOTHERAPY IN AN ENDEMIC AREA OF SCHISTOSOMIASIS JAPONICA

[目的 ]观察日本血吸虫病流行区人群的细胞免疫应答状态及吡喹酮治疗对其影响。 [方法 ]采集日本血吸虫病流行区 12 9人 (粪检阳性者 6 4例 ,粪检阴性者 6 5例 ) ,吡喹酮治疗前及治疗后 45d的静脉血 ,以血吸虫成虫和虫卵抗原分别刺激诱生细胞因子 ,检测培养上清中的细胞因子IL 5、IL 10和IFN γ水平。 [结果 ]12 9例中 ,对血吸虫抗原刺激诱生的细胞因子水平粪检阴性组明显高于粪检阳性组 ;治疗后 ,细胞因子诱生水平较治疗前显著升高 ,特别是IL 5和IFN γ。 [结论 ]流行区人群的细胞免疫应答显示总体下调趋势 ;吡喹酮治疗后出现细胞因子水平的明显升高。

Objective] To observe the cellular immune responses in a population of an endemic area of schistosomiasis japonica and the influence of praziquantel treatment.[Methods] Blood was taken from 129 residents (64 cases were egg positive,65 cases were egg negitive) of an endemic area of Poyang Lake before and 45 days after praziquantel treatment. Cytokines induced by the schistosome soluble egg antigen (SEA) and soluble worm antigen preparations (SWAP) in the peripheral blood cells including IL 5, IL 10 and IFN γ were measured. [Results] Among 129 cases, the cytokine levels were found much higher in egg negative individuals than in egg positive individuals.The cytokine levels induced by both antigens were increased significantly after praziqantel treatment especially IL 5 and IFN γ.[Conclusion] The cellular immune responses in the population in schistosomiasis japonica endemic area exhibited a general trend of down regulation and were elevated significantly after praziquantel treatment.