肝片吸虫和猪蛔虫谷胱甘肽S转移酶免疫小鼠对日本血吸虫尾蚴攻击感染的保护力

THE PROTECTIVE IMMUNITY IN MICE IMMUNIZED WITH FhGST OR AsGST AGAINST SCHISTOSOMA JAPONICUM CERCARIAE

[目的 ]观察天然肝片吸虫谷胱甘肽转移酶 (FhGST)和猪蛔虫谷胱甘肽转移酶 (AsGST)免疫的小鼠对日本血吸虫大陆株尾蚴攻击感染的保护力。[方法 ]从肝片吸虫和猪蛔虫中提取天然谷胱甘肽转移酶 (GST) ,免疫 3次 ,间隔时间为 14d和 7d。第 3次免疫后 5d经腹部皮肤攻击感染大陆株日本血吸虫尾蚴 30条 鼠。感染6wk后用肝门静脉灌注法收集成虫 ,分别计数肝脏、脾脏和大肠组织沉积虫卵数 ,并计算减虫率和减卵率。[结果 ]3个免疫组 (FhGST、AsGST和日本血吸虫rGST)分别与佐剂对照组和空白对照组比较的减虫率分别为2 7 8%、 37 9%、 33 1%和 31 3%、 41 0 %、 36 4% (P <0 0 1)。肝脏的EPG减卵率为 13 5 %～ 17 7% (佐剂对照组 ,P <0 0 5 )和 2 3 9%～ 2 7 6 % (空白对照 ,P <0 0 1)。脾脏的EPG减卵率为 30 4%～ 5 9 6 % (P <0 0 5 ) ,大肠的为 38 7%～ 6 2 3% (P <0 0 1)。 [结论 ]FhGST和AsGST具有明显抗血吸虫感染的保护力。

Objective] To study the protective immunity against Schistosoma japonicum (Sj) cercariae challenge in mice immunized with FhGST or AsGST. [Methods] Three groups of Kunming mice were immunized three times with rSjGST, FhGST and AsGST, respectively. Then 30 cercariae of Schistosoma japonicum were given per mouse by abdominal skin inoculation. Six weeks later, all mice were killed to collect adult worms, liver, spleen and large intestine for worm count and egg count. [Results] The worm burden was reduced by 27 8%～36 4% in the three immune groups compared with the two control groups ( P <0 05). rSj26GST, FhGST and AsGST significantly decreased the number of eggs deposited in the tissues. [Conclusion]: FhGST and AsGST could induce protection against S japonicum cercariae infection.