三种凝集试验检测弓形虫抗体的定性和定量比较

QUALITATIVE AND QUANTITATIVE COMPARISON OF THREE AGGLUTINATION TESTS FOR DETECTING TOXOPLASMA GONDII ANTIBODIES

[目的 ]评价用于检测弓形虫抗体的我室改良凝集试验 (MAT 1)、日本的胶乳凝集试验 (LAT)和法国的改良凝集试验 (MAT 2 )的实用价值。 [方法 ]用 3种试验对 2 88份人血清进行检测 ,比较阳性检出率与符合率等 ,并对检测的半定量结果进行统计分析。 [结果 ]3种试验的阳性率 (9 7%、 8 9%和 8 1% )无显著性差异 (χ2 =0 392 ,P >0 0 5 )。平行检测相同对象的结果显示 :MAT 1与LAT的总符合率为 93 7% ,两法的相关系数r =0 6 13;LAT与MAT 2的总符合率为 94 5 % ,两法的相关系数r =0 5 5 1;MAT 2与MAT 1的总符合率为97 3% ,两法的相关系数r =0 841。应用配对资料的卡方检验进行差异的显著性检验 ,结果均无显著性意义(P >0 0 5 )。而且 ,3种凝集试验中任两法间的正相关均具有非常显著性意义 (P <0 0 0 1)。 [结论 ]检测弓形虫抗体的MAT 1、LAT和MAT 2具有很好的符合性 ,提示 3种方法用于常规普筛和血清流行病学研究时可以相互替代。

Objective] To evaluate the diagnostic value of three agglutination tests used in three countries for detection of antibodies to Toxoplasma gondii .[Methods] A total of 288 human serum samples were assayed using modified agglutination test (MAT 1,using selfmade antigen),latex agglutination test (LAT,using Japanese Kit) and modified agglutination test (MAT 2,using French antigen).[Results] The positive rates of MAT 1 (≥1∶20), LAT (≥1∶32) and MAT 2 (≥1∶20) were 9 7% (28/288), 8 9% (10/112) and 8 1% (17/210), respectively. No significant statistical difference was found among these positive rates (χ 2 =0 392, P >0 05). High agreements were found between MAT 1 and LAT (93 7%), LAT and MAT 2 (94 5%), and MAT 2 and MAT 1 (97 3%). Significant correlation were demonstrated in MAT 1 and LAT (r = 0 613), LAT and MAT 2 (r = 0 551), and MAT 2 and MAT 1 (r = 0 841), p <0 001.[Conclusion] The detection efficiency of the three agglutination tests is in good agreement and could alternatively be used for the diagnosis of toxoplasmosis.