maspin在食管鳞状细胞癌中的表达及其与微血管密度的关系

Expression of maspin in esophageal squamous cell carcinoma and its relationship to mircovessel density

目的研究旨在寻找能预测食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)浸润、转移及术后生存率的生物因子。方法采用免疫组织化学ElivisionTM plus法检测100例ESCC和30例正常食管组织中maspin的表达和微血管密度(microvessel density,MVD)情况。结果在正常食管组织和ESCC组织中,maspin的阳性表达率分别为100%和43.0%,差异有显著性(P<0.01);maspin的表达水平与肿瘤的分化程度、浸润深度、淋巴结转移和临床分期有关(全部P<0.01);MVD计数与肿瘤的大小、分化程度、淋巴结转移以及临床分期有关(全部P<0.01);且maspin的表达与MVD计数呈负相关(P<0.01)。结论 maspin的表达和MVD计数与ESCC组织的分化程度、转移和预后等均有关;maspin和MVD联合检测对ESCC的进展及预后判断有重要意义。

Objective To find markers to predict invasion and metastasis of esophageal squamous cell carcinoma （ESCC）. Methods Maspin expression and microvessel density （MVD） were examined by immu- nohistochemistry in 100 specimens of ESCC and 30 specimens of normal esophageal tissue. Results The positive rates of maspin in ESCC and normal esophageal tissue were 43.0~ and 100~, respectively, with a significant difference （P〈0.01）. The expression of maspin was significantly related with tumor differen tiation, invasion, lymph node metastasis, and pathological-TNM （pTNM） stage （all P〈0.01）; the scores of MVD were significantly related with diameter of tumor, tumor differentiation, invasion, lymph node metastasis, and pTNM stage （all P〈0.01）. The overall mean survival time of patients with maspin negative expression was lower than that of patients with positive expression; the overall mean survival time of patients with MVD≥20 was lower than that of patients with MVD%20. Multivariate analysis showed that： pTNM stage, lymph node metastasis, positive expression of maspin, and MVD were inde pendent prognostic factors of ESCC （P〈0.05）. Conclusion MVD and maspin expression are related to dif ferentiation, invasion, lymph node metastasis, pTNM stage, and prognosis. It is suggested that maspin and MVD should be considered as a potential marker for the prognosis.