VEGF和MVD在下咽鳞癌组织中的表达及其意义

Expression of VEGF and MVD in hypopharyngeal squamous cell carcinoma and their significances

目的:研究VEGF和CD105标记的MVD在下咽鳞状细胞癌中的表达,并研究其与肿瘤临床特性的关系,探讨两者在下咽鳞状细胞癌发生、发展过程中的作用及其相关性。方法:采用免疫组化PV-9000二步法,检测50例下咽鳞状细胞癌及50例正常状况下咽黏膜中的VEGF和CD105标记的MVD的表达水平,应用Image-ProPlus图像分析软件测定免疫组化图像的平均光密度(mean optical density,MOD)。结果:VEGF蛋白在下咽鳞状细胞癌及正常下咽黏膜中的表达率分别为74.00%和30.00%,差异有统计学意义(P=0.002),VEGF蛋白的表达在不同TNM分期、组织学分级和是否淋巴转移组间的阳性表达差异有统计学意义,均P<0.05;CD105标记的MVD在下咽鳞癌组织中的计数(37.10±5.95)显著高于正常下咽黏膜(8.70±3.34),P=0.000,MVD计数在不同TNM分期、组织学分级和是否淋巴转移组间的阳性表达差异具有统计学意义,均P<0.05;VEGF、MVD在不同年龄、性别、吸烟史和原发部位的下咽鳞癌组别间,表达均差异无统计学意义,P>0.05;下咽鳞癌中VEGF表达和MVD计数呈显著正相关(r=0.582,P=0.000)。结论:VEGF和MVD的高表达提示两者对肿瘤新生血管的形成起重要作用;同时,VEGF和MVD在下咽鳞状细胞癌组织中的高表达具有显著正相关性,表明两者协同促进下咽鳞癌的生长和进展。联合检测VEGF和MVD可能对综合评估下咽鳞癌的生物学特性和临床特点有指导意义。

Objective：To detect the expression of VEGF and MVD in hypopharyngeal squamous cell carcinoma, explore their associa- tion with elinicopathological characteristics and role in the development and progression of Hypopharyngeal Squamous Cell Carcinoma as well as their interrelationships. Methods： The expression of VEGF and MVD in 50 specimens of Hypopharyngeal Squamous Cell Carci- noma and 50 specimens of normal hypopharyngeal tissues were evaluated by immunohistochemistry PV-9000 two-step methods. Image- ProPlus software was used to determine the mean optical density （MOD） of immunohistochemical Image. Results： The overexpression rate of VEGF was significantly higher in hypopharyngeal squamous cell carcinoma （74.00%） than in normal hypopharyngeal tissues （30.00%） ,P = 0. 001, and its overexpression showed statistical differences in different TNM stage, pathological grade and cervical lymphnode metastasis groups （P 〈 0.05 ） ;The counting of MVD-CD105 was significantly higher in hypopharyngeal squamous cell carcino- ma（37.10 ± 5.95 ） than in normal hypopharyngeal tissues （ 35.00% ） ,P = 0. 000, and between different TNM stage, pathological grade and cervical lymphnode metastasis groups,its counting showed statistical differences （ P 〈 0.05 ） ;The expressions of two factors were not significantly different in groups of different ages,genders,tabacco hobby, and the primary site of tumor（ P 〉 0.05 ） ; The expression ofVEGF was positively associated with the counting of MVD in hypopharyngeal squamous cell carcinoma（ r = 0. 382 ,P = 0. 000）. Conclu- sion： VEGF,MVD may play important roles in tumor angiogenesis; meanwhile, highly positive correlation between VEGF and MVI） may cooperatively promote tumor growth and progression; combined detection of VEGF and MVD might be significantly helpful to com- prehensively assess the biological characteristics and clinical features of hypopharyngeal squamous cell carcinoma.