儿童孢子丝菌病的临床分析及致病菌的基因型检测

Clinical analysis and genotype identification of pathogenic strains of children 's sporotrichosis in Fuyu county,Jilin Province

目的对吉林省扶余县35例儿童孢子丝菌病进行临床分析并对部分菌株进行基因测定,了解该地区孢子丝菌病的致病菌菌株基因是否发生变异而致致病力增强及发病率增高。方法收集2011年1月1日～4月30日来自扶余县就诊于我科并确诊为孢子丝菌病的患儿35例,进行临床分析;从中选取8株菌,对其核糖体保守区及内转录间隔区(ITS)、非转录间隔区(NTS)区进行基因测定,了解基因水平上的异同。结果 8株孢子丝菌菌株中5株NTS区可见较大范围的碱基缺失。结论吉林省扶余县孢子丝菌菌株可能由于NTS区碱基较大范围缺失而致致病力增强,导致该地区短时间内发病率增高。

Objective To make the clinical analysis of children＇s Sporothrix schenckii infections and genic identification of partial strains in Fuyu county,Jilin Province. And to understand whether the strains in this area had stronger pathogenicity because of genic differences by comparing the gene sequences of these strains with those of Sporothrix schenckii strains from another county in Jilin Province.Methods To analyze the clinical characteristics, 35 cases of children＇s sporotrichosis in our clinic from January 1 to Apirl 30,2011 were enrolled. The genic identifications of ribosome-conservative spacer, ITS and NTS of 8 of the strains were performed.Results Comparing with the standard strain, the NTS of five strains which caused Sporothrix schenckii infections in Fuyu coutry have the loss of many bases. Among them, four strains have the non-contiguous loss of 10 bases from exactly the same positions 208 to 232bp; the other one strain have the non-contiguous loss of 21 bases from the position 208 to 249 bp.Conclusions The strains which resulted in the higher incidence of sporotrichosis in Fuyu county might have stronger pathogenicity because of the bases loss in NTS.