葡萄籽寡聚体原花青素对大鼠酒精性肝损伤及脑功能障碍的保护作用

Oligomeric proanthocyanidins from grape seeds protect against alcohol-induced liver injury and cerebral dysfunction in rats

目的:研究葡萄籽寡聚体原花青素(oligomeric proanthocyanidins,OPC)对大鼠酒精性肝损伤及脑功能障碍的保护作用.方法:将31只SD大鼠随机分为4组.A组为生理盐水组,B组为OPC组,C组为OPC+酒精组及D组为酒精组.2次/d灌胃法,连续灌胃23d.观察动物醉酒潜伏期,血浆天门冬氨酸氨基转氨酶(aspartate aminotransferase,AST)和丙氨酸氨基转氨酶(alanine aminotransferase,ALT)含量以及肝脏和脑组织病理组织学改变.结果:(1)AST和ALT在A、B及C组之间比较,差别都没有统计学意义(P>0.05);D组与其他3组比较,AST和ALT都显著升高,差别有统计学意义(P<0.001);(2)A组及B组肝脏组织均未见明显病理改变.C组及D组大鼠肝脏损伤发生率分别为57.1%及100%;肝组织损伤面积分别为3.6%±3.2%及63%±28%,差异有统计学意义(P<0.001);(3)C组比D组大鼠平均醉酒潜伏期时间明显延长(P<0.0001);(4)4组大鼠脑组织在光镜下均未见明显病理改变.结论:葡萄籽寡聚体原花青素对大鼠酒精性肝损伤具有明显的保护作用,还有抗醉酒作用.

AIM： To study the protective effects of oligomeric proanthocyanidins （OPC） from grape seeds against alcohol-induced liver injury and cerebral dysfunction in rats.METHODS： Thirty-one Sprague-Dawley rats were randomly divided into four groups： A （treated with normal saline）, B （treated with OPC）, C （treated with OPC and alcohol）, and D （treated with alcohol）. Groups A and D were administered intragastrically with 0.9% NaC1 [10 mL/（kg·d）], while groups B and C were administered with OPC solution [200 mg/（kg·d]. After three hours, groups A and B were intragastrically given 0.9% NaC1 groups C and D were [10 mL/（kg·d）], while given 55% alcohol [10 mL/（kg·d）]. After 23 d, blood samples were collected from all animals via the inferior vena cava under general anesthesia, and liver and brain tissue samples were taken and fixed in 10% buffer formaldehyde. The level of aspartate aminotransferase （AST） and alanine aminotransferase （ALT） in the plasma was measured, and the histopathology of the liver and brain was assessed under an optical microscope.RESULTS： Plasma levels of AST were 110.00 U/L ± 15.55 U/L, 98.38 U/L ± 17.86 U/L, 100.14 U/L ± 14.46 U/L and 176.00 U/L ± 49.60 U/L in groups A, B, C and D, respectively, and those of ALT were 57.25 U/L ± 9.04 U/L, 49.50 U/L ± 6.67 U/L, 50.28 U/L ± 5.37 U/L and 74.50 ± 9.69 in groups A, B, C and D. Both AST and ALT levels did not significantly differ between groups A,B and C （all P 〉 0.05）; however, both AST and ALT levels were significantly elevated in group D compared to groups A, B, and C （all P 〈 0.001）. No significant liver injury was found in groups A and B. The incidence of liver injury in group C was lower than that in group D （57.1% vs 100%, P = 0.077）, and the area of injured liver was significantly less in group C than in group D （3.6%± 3.2% vs 63% ± 28%, P 〈 0.001）. The latent periods from alcohol administration to the onset of drunkenness was significantly longer in group C than in group D （16.43 min ± 2.71 min vs 10.67 min ± 2.38 min, P 〈 0.0001）. No significant brain injury was found in all four groups by optical microscopy.CONCLUSION： Pretreatment with OPC provides excellent protection against alcohol-induced liver injury and slows the onset of drunkenness in rats. 2013 Baishideng. All rights reserved.