microRNA-183家族的表达在肝细胞癌临床诊断中的价值

Diagnostic value of expression of microRNA-183 family members in hepatocellular carcinoma

目的:探讨肝细胞癌(hepatocellular carcinoma,HCC)组织及血清中microRNA-183家族的表达与诊断价值.方法:采用实时荧光定量PCR法,检测以自身对照法HCC患者组织(癌、癌周和远癌)和血清中miR-96、miR-182及miR-183的表达,并与肝硬化、慢性乙型肝炎(chronic hepatitis B,CHB)患者及健康体检者进行比较.结果:HCC患者的癌组织和血清miR-96和miR-182表达量均明显高于其他组(P<0.001);miR-183癌组织相对表达量显著低于远癌及癌周(P<0.001),但在血清中各组间差异无统计学意义.HCC患者组织及血清miR-183家族表达呈明显正相关(rmiR-96=0.815、rmiR-182=0.806、rmiR-183=0.851).血清miR-96、miR-182ROC曲线下面积分别为0.927和0.940.HCC患者血清miR-183相对表达量与肝外转移相关(P=0.019).结论:HCC组织miR-183家族呈异常表达,呈现miR-96和miR-182升高,miR-183下降;血miR-96和miR-182异常表达有助于肝癌诊断,而miR-183降低与HCC转移相关.

AIM： To investigate the expression ot mxcroRNA-183 family members in hepatocellular carcinoma （HCC） and to analyze their diagnostic value.METHODS： The expression of miR-183 family members （miR-96, miR-182 and miR-183） was examined by real-time reverse transcriptionquantitative polymerase chain reaction （RTqPCR） in HCC and matched tumor-adjacent and tumor-distant tissues, and in 80 serum samples from patients with HCC, cirrhosis or chronic hepatitis B, and health controls. RESULTS： The levels of miR-96 and miR-182 expression in tissues and sera of HCC patients were significant higher than those in any of other groups （all P 〈 0.001）. The level of miR-183 in the liver was significantly lower in HCC than in matched tumor-adjacent and tumor-distant tissue （both P 〈 0.001）, although there was no significant difference in serum miR-183 between each group. Liver expression of miR-183 family members was positively associated with their serum levels （rmiR-96=0.815、rmiR-182=0.806、rmiR-183=0.851） in HCC. The areas under the receiver operating characteristic curve of serum miR-96 and miR-182 were 0.927 and 0.940. The relative level of serum miR-183 was significantly higher in patients with extrahepatic metastasis than in those without extrahepatic metastasis （P 〈 0.019）.CONCLUSION： Expression of miR-96 and miR-182 is up-regulated and that of miR-183 down-regulated in HCC. Circulating miR-96 and miR-182 are useful biomarkers for HCC diagnosis. Down-regulation of miR-183 is associated with HCC extrahepatic metastasis. 2013 Baishideng. All rights reserved.