新型TiO_2纳米管透析膜与传统透析膜对细胞生长状态的影响

Effect of Novel TiO2Nanotube Dialysis and Traditional Dialysis Films on Cells Growth State and Their Comparison

研究采用阳极氧化法制备新型高强度的TiO2纳米管阵列薄膜,通过对纳米管底部进行腐蚀获得两端通透的TiO2纳米管阵列薄膜。在纳米管阵列薄膜表面和高分子透析膜表面种植HK-2细胞和HUVEC细胞,成功制备具有生理功能的生物膜材料。采用MTT方法对比研究了TiO2纳米管阵列薄膜、聚醚砜(PES)、混合纤维素以及再生纤维素4种薄膜材料黏附细胞的活性;利用荧光显微镜观察4种材料对两种细胞黏附的影响;同时使用扫描电镜观察细胞在4种薄膜材料上的生长形态。结果表明,TiO2纳米管阵列薄膜最有利于细胞的黏附及增殖,且细胞活性最高,PES薄膜的效果次之,再生纤维素薄膜最不适合细胞的增殖及黏附;荧光显微镜观察证实TiO2纳米管阵列薄膜相比高分子薄膜更能促进细胞的黏附及生长,证实所制备的TiO2纳米管阵列薄膜能够很好地与两种细胞相容,克服了传统透析材料的不足,改善了细胞与材料的黏附,是用于生物人工肾研究较为理想的候选材料。

Novel high intensity TiO2 nanotube array film was fabricated via electrochemical anodization, and then open-ended TiO2 nanotubes array membrane was obtained by HF gas etching the bottom of nanotubes. HK-2 cells and HUVEC cells were cultivated on the open-ended TiO2 nanotube array surface and polymer dialysis film surface, and biomembrane materials which have physiological function are successfully achieved. Cell viability of TiO2nanotube array, Polyether Sulfone （PES）, mixed cellulose ester, and regenerated cellulose was investigated by using MTT method. Cell morphology on these membranes was examined by using fluorescence microscope and Filed Emission Scanning Electron Microscope （FESEM）. The results indicate that TiO2nanotube array film is of the most benefit to cell adhesion and cell proliferation, and the cell viability is the highest, and the second place belongs to the PES membrane, the worst case is regenerated cellulose that is unsuitable for cell proliferation and cell adhesion. Fluorescence microscope shows that TiO2 nanotube array membrane could promote the cell adhesion and cell proliferation batter than polymeric membrane does. It demonstrates that TiO2 nanotube array film has excellent biocompatibility, overcoming the deficiency of traditional dialysis materials, improving the cell adhesive;and the film is the ideal candidate materials for biological dialysis.