大气细颗粒物对自发性高血压大鼠心血管系统的影响

Effects of Airborne Fine Particulate Matters on Cardiovascular System of Spontaneously Hypertensive Rats

[目的]观察气管滴注大气细颗粒物(PM2.5)对自发性高血压大鼠(SHR)心血管系统的影响。[方法]将24只雄性SPF大鼠随机分为生理盐水对照组及PM2.5染毒低、中、高剂量(分别为1.6、8.0、40.0mg/kg体重)组,各剂量组均经气管滴注染毒3d。末次染毒24h后,监测记录大鼠的血压、心率和心电图,取腹主动脉血测定大鼠血浆内皮素1(ET-1)、P-选择素和D-二聚体浓度。[结果]与对照组相比,PM2.5各染毒剂量组血压和心率均升高(P<0.05),心率变异性降低(P<0.05),大鼠血浆ET-1水平明显升高(P<0.05)。中、高剂量PM2.5暴露可引起大鼠血浆P-选择素和D-二聚体水平明显升高(P<0.05)。[结论]PM2.5气管滴注可导致大鼠心脏自主神经功能异常并改变凝血纤溶的平衡。

[ Objective ] To study changes in cardiovascular system of spontaneously hypertensive rats exposed to fine particulate matters （PM2.s）. [ Methods ] Twenty-four SPF male spontaneously hypertensive rats were randomly divided into 4 groups including 1 saline control group and 3 PMz5 exposure groups （1.6, 8.0, and 40.0mg/kg body weight, respectively）. Each exposure group received intratracheal instillation once per day for 3 consecutive days. At the 24 h after the last administration, blood pressure, heart rate, and cardiogram were recorded, and blood samples were collected to analyze the concentration of plasma endothelin 1 （ET-1）, P-selection, and D-dimmer. [ Results ] The blood pressure and the heart rate increased significantly and the heart rate variability decreased significantly in the PMzs-treated rats compared with the control group （P〈0.05）. Elevated plasma ET-1 levels were observed in the 3 PMz5 exposure groups （P〈0.05）. The P-selection and the D-dimmer increased significantly in the 8.0 and 40.0mg/kg PMz5 exposure groups （P〈0.05）. [ Conclusion ] Exposure to PMz5 induce changes in autonomic nervous function and blood coagulation ＆ fibrinolysis in rats.