酒花黄酮的纯化工艺

Purification of Total Flavonoids from Hop (Humulus lupulus L.)

目的:研究酒花黄酮纯化工艺。方法:以芦丁为对照品,采用NaNO2-Al(NO3)3-NaOH比色法测定酒花黄酮的含量。比较9种不同大孔吸附树脂对酒花黄酮的静态吸附和解吸作用,并考察供试品溶液pH值、洗脱剂pH值、供试品溶液黄酮质量浓度、流速、洗脱剂体积分数及用量等因素对聚酰胺纯化酒花黄酮的影响。结果:聚酰胺最适于酒花黄酮的纯化,其最佳纯化工艺为:精密称取聚酰胺1g,装入层析柱中,精密移取黄酮质量浓度为2.24mg/mL的供试品溶液10mL,以1.0mL/min的流速上样,洗脱剂为80%甲醇溶液,洗脱体积为8BV(聚酰胺柱体积)。结论:在上述纯化工艺条件下,酒花黄酮纯度为50.05%,得率为32.69%。

The purificaiton of total flavonoids from hop （Humulus lupulus L.） was investigated. Colorimetric determination of total flavonoids was carried out with NaNO2-Al（NO3）3-NaOH. We compared the efficiencies of nine types of macroporous adsorption resin for static adsorption and desorption of hop flavonoids. In addition, the effects of pH, concentration and flow rate of sample and pH, concentration and dose of eluent on the purificaiton efficiency of flavonoids were examined. Polyamide was identified as the most appropriate resin for purifying total flavonoids from hop. The optimal purification conditions were obtained when 10 mL of sample was loaded onto an 1-g polyamide column at a flow rate of 1.0 mL/min followed by desorption with 8 BV of 80% methanol. Under these conditions, the yield of flavonoids from hop was 32.69% with a purity of 50.05%.