辣椒素分子印迹聚合物的制备及其在固相萃取中的应用

Preparation of Capsaicin Molecular Imprinting Polymers and Application to Solid Phase Extraction

以辣椒素为模板分子,采用本体聚合法合成以2-乙烯吡啶(2-VP)、丙烯酰胺(AM)为功能单体的辣椒素分子印迹聚合物(MIPs)。采用平衡结合实验评价其对辣椒素的特异性吸附能力;吸附动力学实验评价其对辣椒素的饱和吸附时间;选择性吸附实验评价其对辣椒素类似物的选择性吸附能力。结果表明,当辣椒素与2-乙烯吡啶、丙烯酰胺的物质的量比为1:2:2时,分子印迹聚合物对辣椒素的吸附效果最佳,其吸附容量为20.46mg/g,识别因子α=3.05;饱和吸附时间为6h;且对辣椒素有较好的选择吸附性。采用分子印迹聚合物对辣椒素进行固相萃取,考察极性不同的淋洗剂对辣椒素特异性吸附和非特异性吸附的洗脱效果,发现以极性较小的氯仿作为淋洗剂时能较大限度保留MIPs固相萃取柱中辣椒素的特异性吸附,并除去非特异性吸附。

Molecular imprinting polymers （MIPs） were synthesized by bulk polymerization, using capsaicin as the template molecule and 2-vinylpyridine （2-VP） and Acrylamide （AM） as the function monomers. The specific adsorption ability of the molecularly imprinted polymers （MIPs） to capsaicin was evaluated by equilibrium binding experiments, saturated adsorption time was assessed by saturated adsorption experiments and selective adsorption ability to eapsaicin analogs was appraised by selective adsorption experiments. The results showed that MIPs exerted the best adsorption efficiency to capsaicin when the ratio of eapsaiein：2-VP：AM was 1：2：2, with a adsorption capacity of 20.46 mg/g and a recognition factor of 3.05. Morevoer saturated adsorption time was 6 h and better selective recognition to capsaiein was found. In this study, the elution efficiencies of eluents of different polarity for specific adsorption and non-specific adsorption of capsaicin in solid phace extraction column of MIPs were investigated. The specific adsorption was kept while the non-specific adsorption was discharged to the greatest extent when using chloroform as the eluent.