糖化酶型时间-温度指示器反应体系的制备

Preparation of Time-Temperature Indicator Reaction System Based on Glucoamylase

利用糖化酶与淀粉的水解反应,以碘液为指示剂,通过颜色变化可以显示时间和温度的累积效应。确定糖化酶型时间-温度指示器反应体系的参数为:以10mL质量浓度40g/L的麦芽糊精为反应底物,3mL质量浓度为1g/L的碘液,糖化酶的加入量是可变的。该时间-温度指示器可应用于冷链运输时间不同的产品。加酶量为50μL的反应体系适用于3～4d的4℃左右的低温温控包装中,通过测定其在不同温度条件下的反应速率,确定该反应体系的活化能为75.94kJ/mol。该体系可以应用于因脂肪氧化而造成质量损失的冷鲜肉等产品。

Color changes occuring during the course of reaction between glucoamylase and starch in the presence of iodine solution as an indicator display the cumulative effects of time and temperature. A time-temperature indicator reaction system was developed with 10 mL of 40 g/L maltodextrin as reaction substrate, 3 mL of 1 g/L iodine solution and variable amounts of glucoamylase. The time-temperature indicator was widely applicable for products at different times of cold chain transportation. The reaction system with 50 μL of glucoamylase was suitable for low temperature controlled packaging for 3 to 4 d at about 4 ℃, and the activation energy was found to be 75.94 kJ/mol. This system had potential application for chilled fresh meat, which is vulnerable to quality deterioration caused by lipid peroxidation.