胶体金免疫层析试纸条定量检测猪尿中克伦特罗

Development of Colloidal-Gold Immunochromatographic Strip for Rapid and Quantitative Detection of Clenbuterol in Swine Urine

目的:建立胶体金免疫层析试纸条快速、定量检测尿样中克伦特罗的方法。方法:采用正交设计获得克伦特罗胶体金试纸条最佳工艺条件,建立T/C比值法定量检测尿样中克伦特罗残留。结果:胶体金免疫层析试纸条检测尿样中克伦特罗的最低检测限0.22ng/mL;当尿液中克伦特罗质量浓度在0.1～2.0ng/mL范围内,胶体金免疫层析试纸条具有较好的线性关系且50%抑制质量浓度(IC50)为0.36ng/mL;加标回收实验结果表明,阴性尿样分别添加0.5、1.0、1.5ng/mL克伦特罗,试纸条检测的平均回收率为(102.4±7.8)%、(96.5±6.8)%以及(103.3±7.2)%。结论:成功建立了基于胶体金免疫层析试纸条定量检测尿样中克伦特罗的方法。

We report on the development of a rapid and quantitative colloidal gold immunochromatographic strip for clenbuterol （CLE） detection in swine urine. The parameters that influenced the assay performance of immunochromatographic strip were optimized by an orthogonal array design L9 （3）3 and a quantitative method based on T/C ratio was developed to detect CLE residue in swine urine. The limit of detection （LOD） of immunochromatographic strip for CLE in swine urine was 0.22 ng/mL and a linear calibration curve was obtained over the concentration range of 0.1 to 2.0 ng/mL with a half maximal inhibitory concentration （IC50） of 0.36 ng/mL. For spiked blank urines, mean recoveries were （102.4 ± 7.8）% at 0.5 ng/mL, （96.5 ±6.8）% at 1.0 ng/mL and （103.3 ± 7.2）% at 1.5 ng/mL, respectively. In conclusion, we have successfully developed a rapid and quantitative method based on colloidal gold immunochromatographic strip for CLE residue in swine urine.