高效液相色谱法测定东盟水果中氟啶脲残留量

Development of a High-Performance Liquid Chromatographic Method for Detection of Chlorfluazuron Residues in Fruits Cultivated in Southeast Asian

建立东盟水果中氟啶脲残留的检测方法,以香蕉、荔枝和芒果为材料,利用高效液相色谱法对东盟水果中氟啶脲进行研究。采用丙酮提取样品,提取液经弗罗里硅土柱净化,用高效液相色谱仪结合紫外检测器进行测定,外标法定量。结果表明:氟啶脲在0.05～5.00mg/L质量浓度范围内线性关系良好,相关系数为0.99996;该方法的添加回收率在84.7%～102%之间,变异系数在2.0%～6.7%之间,最低定量限为0.02mg/kg。建立的氟啶脲残留量测定方法满足国家标准对食品中农药残留测定的要求,可用于相关农产品及食品中苯酰脲类农药的质量监控。

A analytical method was established to determine chlorfluazuron residues in banana, mango and litchi cultivated in Southeast Asia by high performance liquid chromatography （HPLC）. Samples were extracted acetone and the extact was then cleaned up on a Florisil column prior to analysis by HPLC with UV detection. The analytical column was an Agilent Eclipse XDB C18 column （4.6 mm × 250 mm, 5 μm） and the mobile phase was methyl alcohol- water （90：10, by volume） at a flow rate of 1.0 mL/min. The detection wavelength was set at 258 nm. Quantification was performed by the external standard method. The results showed that the linear range for chlorfluazuron was in the range of 0.05-- 5.00 mg/L with a correlation coefficient of 0.99996. The average recoveries of chlorfluazuron were between 84.7% and 102%, with a variation coefficient ranging from 2.0% to 6.7%. The limit of quantification of this method was 0.02 mg/kg. This method met the national requirements for pesticide determination and was applicable to food quality supervision and control on chlorfluazuron.