HPLC-ELSD法同时测定食品中的10种糖和糖醇

Simultaneous Determination by HPLC-ELSD of Ten Kinds of Sugars and Sugar Alcohols in Foods

建立高效液相色谱-蒸发光散射法(HPLC-ELSD)同时测定食品中果糖、葡萄糖、蔗糖、麦芽糖和乳糖、赤藓糖醇、木糖醇、山梨糖醇、甘露糖醇、麦芽糖醇的方法。方法采用Grace Previl Carbohydrate ES色谱柱,柱温30℃;乙腈-水为流动相梯度洗脱,流速1.0mL/min;蒸发光散射检测器,飘移管温度95℃,氮气流量2.5L/min。结果表明:5种糖和5种糖醇均在线性0.2～20mg/mL范围内,线性良好,精密度RSD小于5%,回收率91.53%～105.2%。本方法在30min内完成了10种糖和糖醇的分离,灵敏度高,重现性好,可以用于无糖食品中糖和糖醇含量的测定。

A high performance liquid chromatography with evaporative light-scattering detection （HPLC-ELSD） method was developed for the simultaneous determination of 5 sugars including fructose, glucose, sucrose, maltose, lactose and 5 sugar alcohols inlcuding erythritol, xylitol, sorbitol, mannitol, and maltitol in foods. Extracts of samples were separated on a Carbohydrate ES HPLC column at 30 ℃ using acetonitrile-water as mobile phase at a flow rate of 1.0 mL/min. The detector drift tube was kept at 95 ℃ and the nitrogen rate was set at 2.5 L/min. The results showed that the linear range of all these analytes was 0.2-20 mg/mL. The precision of the HPLC-ELSD method was 〈 5% in 6 replicate determinations, and average spike recoveries were 91.53%-105.2%. Successful separation and sensitive and reproducible detection of the sugars and sugar alcohols were achieved by this method within 30 min, thereby providing a promising approach for simultaneous determination of sugar and sugar alcohols in food.