人乳头瘤病毒检测在宫颈病变筛查中的作用

Effect of human papillomavirus detection on screening of cervical lesions

目的了解深圳市罗湖社区已婚育龄妇女人乳头瘤病毒（1tPV）感染的流行状况，探讨HPV检测在宫颈病变筛查中的临床应用价值。方法应用多聚合酶链反应（PCR—MS）HPV分型检测方法，对1002例已婚妇女进行下生殖道HPV型别检测，对其中阳性病例行液基细胞学检查（LCT）检查，对LCT结果为意义不明的不典型鳞状细胞（AS（]US）及以上的病例行阴道镜下活检病理组织学确诊。结果HPV检测阳性61例，阳性率为6．09％。IIPV型别以HPV16、58．18、6、51、52、66型为主。LCT结果为ASCUS及以上的病例28例，阴道镜下活检确诊宫颈上皮内瘤变（CIN）Ⅰ8例，CINⅡ2例，CINⅢ3倒，宫颈早期浸润癌1倒。结论①深圳市罗湖社区已婚妇女中有6．09％存在HPV感染，HPV型别以16、58、18、6、51、52、66型较为多见。②HPV联合LCT检查及阴道镜下活检，能有效梭出宫颈病变病例，这对宫颈上皮内瘤变及宫颈癌的早期诊治具有重要临床应用价值。

Objective To understand human papillomavirus（HPV） infection status in 1002 married women in Luohu community, in Shenzhen, and to investigate the value of HPV detection on screening of ce.rvical lesions. Methods Polymerase chain reaction （PCR） HPV ＂method was used to detect IqPV type,tilt; positive cases were examined by liquid-based cytology test（LCT）. A biopsy under colposcopy was performed in women whose LCT test ≥atypical squamous ceils of determined significance （ASC-US）. Results Sixty-one cases were positive in 1002 cases, the positive rate of HPV infection was 6.09%, ttPV 16, 58, 18, 6, 51, 52 and 66 type were more common. Women whose LCT results ≥ASC-US were 28 cases. Biopsy under colposcope diagnosis of cervical intraepithelial neoplasia（CIN） Ⅰ in 18 cases, ClN Ⅱin 9 cases, CIN Ⅲ in 3 cases,cervical cane.er in 1 case. Conclusions About 6. 09% marfled women in Luohu community, in Shenzhen, have HPV infection, type 16, 58, 18, 6, 51,52 and 66 are more common. HPV combined with LCT and biopsy under colposcopy can effectively detect cervical lesions, that will has important clinical application value in early diagnosis and treatment of cervical intraepithelial neoplasia and cervical cancer.