中华绒螯蟹促雄腺的结构与功能

Structure and function of the androgenic gland in Eriocheir sinensis

应用组织切片、组织化学、电镜及体外培养技术研究了中华绒螯蟹促雄腺的结构与功能。结果表明 ,中华绒螯蟹促雄腺 1对 ,呈不规则索状 ,位于近阴茎基部射精管的表面 ,由许多实心腺泡组成 ;腺体结构与个体精巢发育周期密切相关 ,可明显划分为增殖期、合成期和分泌期 3个发育时期 ;腺细胞以全浆方式分泌激素 ,茚三酮———Schiff反应、磷钼酸法反应呈阳性 ,推断分泌激素中主要含蛋白质和脂类 2种化学成分 ;体外共培养实验证明 ,促雄腺具有促进精子发生与排放及抑制卵母细胞成熟的功能。

Structure and function of the androgenic gland in Chinese mitten-handed crab, Eriocheir sinensis, were studied with tissue section, histochemistry, electron microscope and culture in vitro techniques from April 1996 to March 1997. A pair of androgenic glands were located on the surface of the subterminal portion of the ejaculatory duct near the base of penis. The cords of the gland tissue consisted of many gland alveoluses. The structure of the gland changed greatly with the testis development cycle. The developmental cytology of the gland cells can be distinctly devided into three stages: proliferation phase, synthesis phase and secretion phase. The gland secreted in a holocrine fashion. The androgenic gland hormone is composed primarily of protein and lipid as indicated by positive reaction to Ninhydrin-Schiff and phosphomolybdic acid. Spermatogenesis can be accelerated and oocyte development can be inhibited by the androgenic glands when cultured in vitro.