鲤脑垂体匀浆液和人绒毛膜促性腺激素混合注射对鳗鲡脑区促性腺激素释放激素和血清促性腺激素及性类固醇激素含量的影响

Eeffect of combinative injection of carp pituitary extraction and human chorionic gonadotropin on brain gonadotropin-releasing hormone and serum gonadotropin and sex steroids contents in Anguilla japonica

雄鳗注射 5～ 6次、雌鳗注射 9～ 10次鲤脑垂体匀浆液 (CPE) +人绒毛膜促性腺激素 (HCG)能分别诱导精巢和卵巢发育成熟。在雌雄鳗鲡 ,注射CPE +HCG可显著增加端脑、间脑、中脑和下丘脑mGnRH的含量 ,而对后脑和延髓mGnRH的影响较小 ;注射CPE +HCG增加雄鳗后脑和延髓cGnRH -Ⅱ含量 ,对雌鳗脑区cGnRH -Ⅱ则无显著影响。雌雄鳗鲡每次注射CPE +HCG后 1天 ,血清促性腺激素 (GtH)急剧上升 ,10天后逐渐下降 ,但雌鳗在注射 7次后 10天血清GtH水平仍然显著高于相应对照组。雄鳗注射CPE +HCG 1～ 4次后 ,血清睾酮浓度无显著变化 ;雌鳗每次注射CPE +HCG后 ,血清睾酮浓度升高并一直维持在较高水平。雌鳗血清 17β -雌二醇浓度在注射 4～ 6次期间与对照组无显著差异 ,注射 7次后显著上升。

The testes and ovarian development and maturation were achieved by combinative injection of carp pituitary extraction(CPE) and human chorionic gonadotropin (HCG) for 5-6 times in male and 9-10 times in female Japanese eel(Anguilla japonica). The mGnRH contents in the telencephalon, disencephalon, mesencephalon and hypothalamus significantly increased after injection of CPE+HCG in male and female Japanese eel, but contents in the metencephalon and myelencephalon did not change. Injection of CPE+HCG also increased the cGnRH-Ⅱ contents in the metencephalon and myelencephalon in male Japanese eel, but had no effect in the female.The serum GtH levels in male and female Japanese eel increased dramatically 1 day after each injection of CPE+HCG, then gradually decreased 10 days after. However the serum GtH levels in female Japanese eel remained significantly higher than the corresponding control in 10 days after 7 injections. The serum testosterone concentration increased after injection of CPE+HCG and kept on a high level in female Japanese eel, but did not change during the time of 1-4 injection in male Japanese eel. The serum 17β-estradiol contents were not different with the corresponding controls during 4-6 injections in female Japanese eel, but increased after 7 injections.