纳米二氧化锆超细颗粒物对KB细胞活性的影响

Effect of ZrO_2 Nano-particles on the viability of KB cells in vitro

目的评估纳米二氧化锆(nano-ZrO2)对人类口腔细胞活性的影响。方法采用不同浓度的纳米ZrO2颗粒(0、10、30、60、100、150、250μg.ml-1)对Human epidermoid carcinoma(KB)细胞分别进行24、48、72 h的染毒,应用MTT法检测细胞活性。结果①24、48 h低浓度纳米ZrO2染毒时KB细胞活性较高;②24、48 h高浓度nano-ZrO2染毒时没有对KB细胞的活性产生明显影响;③染毒72 h之后KB细胞活性没有发生显著变化,nano-ZrO2对KB细胞也不具有细胞毒性。结论在适当浓度下,nano-ZrO2对KB细胞活性无明显毒作用。

Biology,College of Life Sciences,Central China Normal University,Wuhan 430079,China Objective To assess the effect of ZrO2 Nano-particles（NPs） on the viability of human oral cells in vitro.Methods Human epidermoid carcinoma（KB） cells were exposed to ZrO2 NPs with different concentrations（0、10、30、 60、100、150、250 μg.ml-1） for 24,48,72 h.MTT assay was used to analyze the viability of KB Cells.Results ① The viability of KB cells was increased by low concentration ZrO2 NPs at 24 and 48 hours’ exposure time.② At the same time,high concentration ZrO2 NPs had no significant impact on the viability of KB cells.③ After 72 hours the activity of KB cells had no significant change,and ZrO2 NPs had no significant toxicity to KB cells too.Conclusions It was suggested that ZrO2 NPs,at the appropriate concentration,had no harmful effect on the viability of the KB cells.