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表位竞争可削弱针对非优势表位的T细胞应答

Epitope competition weakens specific T cell response to subdominant epitopes
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摘要 目的阐明表位竞争对特异性T细胞应答强度和功能特征的影响。方法利用pSV—gag92和pSV-env203两个单表位DNA疫苗和pSV-gag/env融合基因疫苗免疫C57BL/6小鼠。免疫结束后,通过细胞内细胞因子染色的方法检测Gag92和Env203特异性T细胞反应,并比较其差异。结果pSV—gag92所活化的Gag92特异性IFN—γ+CD8T细胞占总CD8T细胞比例[(0.41500±0.04588)%]显著高于pSV—gag/env[(0.05867±0.01964)%]。同时,我们发现pSV—gag/onv所活化的Gag92特异性TNF-α-—IFN-^γ’CD8T细胞平均荧光强度(296.70±14.08)显著低于Env203特异性TNF-(It—IFN-^γ+CD8T细胞的平均荧光强度(818.00±49.34)。结论表位竞争能够显著降低针对非优势表位的T细胞反应频率和平均强度。 Objective To elucidate the influences of epitope competition on the frequency and av- erage intensity of specific T cell response. Methods C57BL/6 mice were immunized with either single epitope DNA vaccines (pSV-gag92 or pSV-env203 ) or fusion gene DNA vaccine (pSV-gag/env). Gag92 and Env203 epitope-speeific CD8 T cell responses were analyzed by intracellular cytokine staining assay. Results Gag92-specific IFN-γCD8 T cells that were induced by pSV-gag92 accounted for 0. 415 00% ± 0.045 88% of the total CD8 T cells, which was much more than that induced by pSV-gag/env of 0.058 67%± 0. 019 64%. Moreover, the mean fluorescence intensity of Gag92-specific TNF-α-IFN-γ/CD8 T cells (296.70±14.08) elicited by pSV-gag/env was significantly lower than that of Env203-specific TNF- α-IFN-γ/+CD8 T cells (818.00±49.34). Conclusion Epitope competition could significantly decrease both the frequency and the average intensity of specific T cell response to subdominant epitopes.
出处 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2013年第6期440-444,共5页 Chinese Journal of Microbiology and Immunology
基金 国家自然科学基金面上项目(81072496) 上海市自然科学基金(11ZR1430600) 中国博士后科学基金特别资助(201003234)
关键词 单表位DNA疫苗 表位竞争 T细胞反应 Single epitope DNA vaccine Epitope competition T cell response
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