腐殖质还原菌的筛选及其对污染物的生物降解

Isolation of a Humus-reducing Bacterium Strain and Its Biodegradation of Pollutants

为研究腐殖质还原菌在污染物生物降解及污染环境生物修复中的作用,在厌氧条件下从巢湖底泥中富集分离到一株腐殖质还原菌,命名为F1.通过电镜镜检、16S rDNA序列分析及还原活性研究,结果表明,F1为克雷伯氏菌,能够以腐殖质模式物蒽醌-2,6-双磺酸钠(AQDS)为唯一电子受体进行厌氧胞外呼吸,还原AQDS的最适温度为25℃,初始pH为8,能较好利用乳糖,对培养液中Cr(Ⅵ)具有很强的还原能力;在添加AQDS时对培养液中的对硝基苯酚(PNP)去除率可以达到81.31%,添加三价铁离子对土壤中PNP降解有一定的促进作用,去除率可以达到93.26%.研究为通过腐殖质微生物还原修复污染环境提供了数据和资料.

In this study, a strain of bacteria, named F 1, was isolated from the sediment of the Chaohu Lake under anaerobic conditions to study the effect of microbial humus reduction on biodegradation of pollutants and biorestoration of contaminated environment. According to the results of electron microscope and 16S rDNA sequencing, F1 was identified as Kleb,~iella. It can utilize anthraquinone-2,6-double sodium sulfonate, the humus mode content, as the only electronic receptor for anaerobic extracellular respiration. The optimal temperature and initial pH is 25 ℃ and 8, respectively, for AQDS reduction of F1. FI can utilize lactose, and has a strong reducibility of Cr （VI） in the nutrient solution. Its degradation rate for p-nitrophenol can be up to 81,31% when added with AQDS, and up to 93.26% when added with ferric iron ion. Fig 9, Tab2, Ref 17