摘要
为了探索植物桫椤内生细菌及其进化关系,对用植物内生菌一般分离培养方法获得的4株内生细菌,从形态学、生理生化特征以及16S rDNA PCR扩增、测序和BLAST比对等进行了综合鉴定和系统发育分析.根据形态学和生理生化特征以及16S rDNA相似性比对,将内生细菌ASP17和ASL21初步鉴定为苏云金芽孢杆菌(Bacillus thuringiensis);将ASL24初步鉴定为Lysinibacillus属,而ASL23可能为与Lysinibacillus相近但不同的属.系统发育分析表明:4株内生细菌中ASP17和ASL21聚为一支,亲缘关系较近;ASL23和ASL24聚为一支,亲缘关系较近.
To determine the endophytic bacteria inhabiting AIsophila spinulosa and their phylogenetic relationship, integrative identification and phylogenetic analysis was made on 4 strains of isolates with traditional microbiological methods. Based on morphological and biochemical properties and sequencing and comparison results with BLAT, the isolates ASP17 and ASL21 were identified as species Bacillus thuringiensis, ASL24 was identified as genus Lysinibacillus, while ASL23 possibly belonged to another genus close to Lysinibacillus. Among the 4 endophytic bacteria, ASP17 and ASL21 clustered in one branch, while ASP23 and ASP24 clustered in another branch in clad0gram. The results revealed that the genetic relationship between ASPI7 and ASL21 is closer than between ASP23 and ASP24. Fig 2, Tab 2, Ref21
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2013年第3期528-531,共4页
Chinese Journal of Applied and Environmental Biology
基金
贵州省科学技术基金项目(20082103)资助~~
关键词
桫椤
内生细菌
16S
RDNA
分离鉴定
系统发育分析
Alsophila spinulosa
bacterial endophytes
16S rDNA
isolation and identification
phylogenetic analysis
