红外光谱法在石斛鉴别中的应用研究进展

Advances in Infrared Spectroscopy Methods for Discriminating Dendrobium

名贵中药石斛原植物来源属于珍稀濒危植物,是多基源(中国记载分布有76种)、同部位入药的中药材,为中国药典中基源收载最为复杂的中药之一,需要简便、快速的方法来对不同基源的石斛进行鉴别.红外光谱法具有快速简便、指纹性强等特点,特别适合中药材这一复杂体系的快速鉴别.本文简要介绍了红外光谱及红外光谱法鉴别中药的原理以及与红外光谱法鉴别中药相关的化学计量学方法的基本原理和特点;综述了近年来红外光谱及其二阶导数谱对比法、二维相关红外光谱法、红外光谱结合化学计量学方法在石斛属植物鉴别中的应用研究进展,研究表明红外光谱鉴别石斛具有简便快速、特征性强、取样量少等优点.随着红外光谱技术本身的发展及其在石斛研究中的广泛应用,该方法将成为石斛鉴别的主要方法之一.

Dendrobium, as a precious Chinese medicine, is a rare and endangered plant. Because the same part of the plant of multiple sources is used for medical purposes, Dendrobium is one of the most complicated entries recorded in Chinese Pharmacopoeia. Therefore, a convenient and fast method spectroscopy is fast, convenient, and highly sensitive is needed to discriminate different species of Dendrobium. Infrared with fingerprint, thus extremely suitable for fast discriminating the complex system of traditional Chinese medicines. This paper introduces infrared spectroscopy and its application in discriminating traditional Chinese medicines, as well as the basic principles and characteristics of infrared spectroscopy- related Chemometrics. We examined the application progresses in discriminating Dendrobium of infrared spectroscopy and derivative methods, including two-dimensional infrared spectroscopy method and infrared spectroscopy-related Chemometrics method, showing that infrared spectroscopy has the advantages of convenience, efficiency, fingerprint and little-dosage in discriminating Dendrobium. With the development of infrared spectroscopy and its extensive use in research on Dendrobium, this technology will become one of the main discriminating methods for Dendrobium discrimination. Ref26