CIK分泌的细胞因子对逆转耐顺铂肺腺癌细胞系A549/DDP耐药性的研究

Cytokines secreted by cytokine-induced killer cells modulate resistance to cisplatin in the A549/DDP cell line

目的:探讨细胞因子诱导的杀伤细胞(cytokine induced killer,CIK)分泌的细胞因子在逆转耐顺铂(DDP)人肺腺癌细胞系A549/DDP顺铂耐药性中的可能作用。方法:采用transwell非接触共培养CIK与A549/DDP细胞,收集不同时间点的共培养上清,酶联免疫试剂方法(ELISA)检测IFN-γ、TNF-α、IL-2的分泌情况,四氮甲唑兰比色法(MTT法)检测DDP耐药性的变化,实时定量PCR检测GST-π基因表达水平的变化。结果:分析发现A549/DDP细胞的耐药逆转与IFN-γ的分泌水平显著相关,与TNF-α、IL-2的分泌量无显著相关。给予anti-IFN-γ中和抗体后,共培养20h A549/DDP的DDP耐药性显著上升(P<0.05),GST-π基因水平的表达量较未加中和抗体组也显著增加(P<0.05)。而加入anti-TNF-α、anti-IL-2中和抗体组,A549/DDP细胞的DDP耐药性及GST-π基因水平的表达量较未加入中和抗体组无明显变化(P>0.05)。结论:CIK通过分泌IFN-γ下调A549/DDP细胞中GST-γ的表达来实现其逆转DDP耐药的作用。

Objective： This study aimed to explore the possible roles of cytokines secreted by cytokine-induced killer （CIK） cells in reversing the DDP resistance of A549/DDR Methods： Supernatants of co-cultured systems were collected to detect the levels of IFN-γ, TNF-α, and IL-2 using enzyme-linked immunosorbent assay （ELISA）. The correlations between cytokines and resistance rever- sal indices were analyzed at different co-cultured time points. Specific neutralizing antibodies against IFN-γ , TNF-α, and IL-2 were added to the co-cultured system to evaluate the effects of the aforementioned eytokines on reversing DDP resistance in A549/DDP, as well as on the expression of GST-π gene. Results： ELISA and correlation analyses showed that IFN-γ was related to the reversal of DDP resistance of A549/DDE After neutralization by anti-IFN-γ antibody, the DDP resistance of A549/DDP co-cultured with CIK for 20 h significantly recovered （P〈0.05） and the expression of GST-π at mRNA level significantly recovered compared with A549/DDP co-cultured without anti-IFN-γ antibody （P〈0.05）. Conclusion： IFN-γ secreted by CIK might decrease the expression of GST-γ in A549/DDP to achieve reversal of DDP resistance.