西洛他唑对脂多糖诱导的人THP-1单核细胞分泌白细胞介素-6的影响及机制

Mechanism and influence of clostazol on LPS-induced THP-1 mononuclear cells secreting IL-6

目的:研究西洛他唑对脂多糖(LPS)诱导的人THP-1单核细胞分泌白细胞介素(IL)-6的影响及机制,探讨西洛他唑对单核细胞的炎症调控作用。方法:体外培养人THP-1单核细胞,分为对照组、LPS组、西洛他唑低浓度组、西洛他唑中浓度组以及西洛他唑高浓度组,西洛他唑各组分别加入不同浓度(10、20、40μmol/L)西洛他唑干预12h,再加入LPS刺激24h。应用ELISA法检测各组细胞培养上清IL-6水平,Westernblot检测各组细胞质IκBα及细胞核因子(NF)-κBP65的表达。结果:西洛他唑呈浓度依赖性抑制LPS刺激的人THP-1单核细胞IL-6水平,减少IκBα的降解及核内NF-κBP65水平表达。结论:西洛他唑可抑制LPS刺激的人THP-1单核细胞分泌IL-6,这种作用可能与抑制NF-κB活化有关。

Objective：To study the mechanism and influence of clostazol on LPS-induced THP-1 mononuclear cells secreting IL-6 and explore the inflammation regulation function of cilostazol on monocytes. Method：The human THP-1 mononuclear cells were cultured and divided into 5 groups： control group, LPS group, and three different concentrations cilostazol groups. After pre-incubated with cilostazol （10, 20, and 40 μmol/L） for 12 h, three cilostazol groups were stimulated with LPS for 24 h. The level of IL-6 was measured by ELISA, and the expression of NF-κB P65 was measured by Western blot. Result：Cilostazol concentration-dependently inhibited IL-6 secretion in human THP-1 monoclear cells induced by LPS, it also reduced the degradation of IκBα and suppressed the expression of NF-κB P65 in nucleus. Conclusion：Cilostazol can suppress IL-6 secreted by human THP-1 mononuclear cells induced by LPS, and the mechanism may be involved with the inhibitory effect on NF-κB activation.