摘要
对我国狂犬病疫苗生产株aG株进行全基因序列测定分析,为完善aG株毒种的质量控制提供数据支持。将aG株病毒全基因组RNA分成8段进行RT-PCR分段扩增,其中基因组5′末端采取5′RACE方法,将PCR扩增产物分别克隆入pGEM-T载体中,测定序列并拼接获得病毒全基因序列;用DNAStar软件包中的相应软件对基因全序列进行分析,并与国内外主要狂犬病疫苗生产株进行基因同源性分析和主要抗原位点比较。aG株病毒基因组序列全长11 925bp(GenBank登录号为JN234411),属基因Ⅰ型狂犬病病毒;各疫苗株生物信息学分析表明,各株病毒存在同源性差异。本研究获得了aG株病毒全基因组序列,对aG株基因特征进行了分析并将其与国内外疫苗株进行了比较,为完善其质量控制提供了参考和数据支持。
To sequence and analyze the full-length gene sequence of rabies vaccine virus aG strain. The full- length gene sequence of aG strain was amplified by RT-PCR by 8 fragments,each PCR product was cloned into vector pGEM-T respectively, sequenced and assemblied; The 5'leader sequence was sequenced with method of 5' RACE. The homology between aG and other rabies vaccine virus was analyzed by using DNAstar and Mega4.0 software, aG strain was 11 925nt(GenBank accession number: JN234411) in length and belonged to the genotype I. The Bioinformatics revealed that the homology showed disparation form different rabies vaccine virus, the full-length gene sequence of rabies vaccine virus aG strain provided a support for perfecting the standard for quality control of virus strains for production of rabies vaccine for human use in China.
出处
《病毒学报》
CAS
CSCD
北大核心
2013年第4期404-409,共6页
Chinese Journal of Virology
基金
国家自然科学基金-基因缺失型减毒狂犬病疫苗构建的基础研究(C080501)
关键词
狂犬病病毒
aG株
全基因组
基因同源性
Rabies virus
aG strain
Full-length gene sequence
Genetic homology