神经生长因子对雪旺细胞增殖的影响

Effects of nerve growth factor on proliferation of Schwann cells

目的:观察神经生长因子(NGF)对体外培养大鼠雪旺细胞(SCs)增殖的影响。方法:采用MTT法观察NGF对SCs增殖的影响及对高糖引起的SCs增殖抑制的保护作用。结果:NGF能够促进SCs的增殖,同时对40 mmol·L-1葡萄糖(Glu)引起的细胞增殖抑制具有明显的改善作用,最佳浓度为100 ng·mL-1。结论:适量浓度的NGF可以促进SCs的增殖,同时对高糖引起的细胞增殖抑制具有明显的改善作用,从而促进周围神经损伤修复。

Objective： To explore the promoting effect of nerve growth factor on proliferation of Schwann cells. Methods： The effects of nerve growth factor on proliferation and high glucose-induced proliferation inhibition were evaluated by MTT reduction assay in Schwann cells. Results： Nerve growth factor promoted the proliferation of Schwann cells, and significantly improved 40 mmol. L -1 glucose-induced proliferation inhibition in Schwann cells. The optimum concentration was 100 ng.mL-1 Conclusion： Nerve growth factor promotes the proliferation of Schwann ceils and improves high glucose-induced proliferation inhibition; thereby can facilitate repair of peripheral nerve injury.