甘蔗黑穗病菌拮抗菌HAS的筛选和鉴定

Screening and Identification of a Biocontrol Strain,HAS Antagonistic to Sporisorium scitaminea Syd

采用传统形态学鉴定及16S rRNA基因序列分析,筛选鉴定对甘蔗黑穗病菌具有拮抗作用的细菌的归属,并采用细菌的通用引物P0、P6扩增16S rRNA基因片段。结果表明:得到1 533 bp的DNA片段,其序列与枯草芽孢杆菌序列的同源性高达99%。其形态学特征:菌体为短杆状,能运动;革兰氏阳性反应;3%KOH溶解性实验呈阴性反应;有鞭毛,鞭毛周生;芽孢中生,椭圆形,孢囊稍膨大,初步将该菌株归属为枯草芽孢杆菌。与病原真菌对峙培养的结果表明,该菌对引起甘蔗和其它作物病害的多个植物病原真菌均具有很好的抑制作用。据此,可将分离的菌株HAS确定为对甘蔗黑穗病菌有较好防治效果的枯草芽孢杆菌。

The present study aims to screen and identify a biocontrol strain, which could antagonize the growth of sugarcane Sporisorium scitaminea Sydow effectively. The sequence of 16S rRNA gene has 99% identity to Bacillus subtilis according to the amplified fragment of 1 533 bp with a pair of universal primers, P0 and P6, of bacteria. From above, HAS strain with short rod, Gram positive, negative reaction of 3% KOH solubility experiment, flagellum around, spore in the middle, elliptic, slightly swelled was identified as B. subtilis based on morphology and biotechnology. It could effectively restrain the growth of sugarcane S. scitaminea. On co-culture plates this strain showed high suppression effect on many fungal pathogens involved in sugarcane and other crops.