香蕉果实乙烯释放量GC的测定方法及其不同处理下的变化趋势

Determination of Ethylene Release Rate in Banana Fruit Using Gas Chromatography

采用毛细管柱气相色谱法测定香蕉果实采后成熟期的乙烯释放量,以外标法峰面积定量,以保留时间定性,乙烯测定结果相对标准偏差(RSD)为1.85%,检出限为0.062μL/L。该气相色谱法测定香蕉乙烯释放量和生成速率具有快捷高效、灵敏度高、重复性好、结果理想等优点。香蕉果实采后不同成熟处理条件下的乙烯释放量跟踪测量中,不同成熟度的香蕉乙烯释放量变化趋势明显,外源乙烯诱导时乙烯释放量提前达到峰值,1-MCP处理下的乙烯释放量只维持在很低水平。

Ethylene, an important phytohormone, plays a significant role in the regulation of fruit ripening. In our research, the endogenous ethylene production in different stages of postharvest banana ripening was measured using capillary gas chromatography （GC）. Their peak areas were determined by the external standard method The relative standard deviation （RSD） of ethylene determination in latex was 1.85%, with the detectable limit of 0.062 μL/L. It was found that the capillary gas chromatography method was not only simple and rapid, but also more accurate and sensitive than other methods in determining the ethylene content and its producing rate in banana fruit. The results showed that the ethylene production of naturally ripening, exogenous ethylene treatment and 1-MCP treatment bananas in the postharvest ripening, showed the similar pattern, in accordance with present research, indicating that this method is a practical and an ideal approach.