鞘脂激活蛋白原(PSAP)在多囊卵巢综合征模型大鼠卵巢中的表达及意义被引量：1

Expression and Significance of Prosaposin (PSAP) in A Rat Model of Polycystic Ovary Syndrome

导出

摘要目的:探讨鞘脂激活蛋白原(PSAP)在多囊卵巢中的表达。方法:用免疫组织化学、RT-PCR和Western blotting方法检测来曲唑(LE)诱导的多囊卵巢综合征(PCOS)大鼠模型卵巢组织的PSAP表达。结果:LE诱导的PCOS大鼠模型内分泌紊乱,代谢特点和形态特征均与人的PCOS相似。在PCOS大鼠卵巢中,PSAPmRNA和蛋白表达均增高。结论:PSAP在LE诱导的PCOS大鼠模型卵巢组织中过表达,并在PCOS发生、发展过程中起到重要的作用。 Objective： To investigate the prosaposin （PSAP） expression in polycystic ovaries. Methods： The expression of PSAP was examined using immunohistochemistry, quantitative RT-PCR and Western blotting in a rat model with letrozole-induced polycystic ovary syndrome （PCOS）. Results： Letrozole-induced PCOS rat models were similar to those in human PCOS in endocrine disturbances, metabolic characteristics and morphology features. And in ovaries of PCOS rat models both mRNA and protein levels of PSAP were increased significantly. Conclusion： PSAP was overexpressed in the letrozole-induced PCOS rat model and might play important roles in occurance and development of PCOS.

作者方媛媛李一宁叶莹心王秀霞

机构地区中国医科大学附属盛京医院妇产科

出处《生殖与避孕》 CAS CSCD 2013年第6期372-377,共6页 Reproduction and Contraception

基金辽宁省教育厅高等学校科研项目项目号:LT2010106

关键词多囊卵巢综合征(PCOS) 雄激素受体(AR) 鞘脂激活蛋白原(PSAP) 大鼠模型 PCOS androgen receptor （AR） prosaposin （PSAP） PCOS rat model

分类号 R711.75 [医药卫生—妇产科学]

引文网络
相关文献

参考文献22

1Ehrmann DA. Polycystic ovary syndrome. N Engl J Med,2005,352(12):1223-36.
2Matalliotakis I,Kourtis A, Koukoura O, et al. Polycysticovary syndrome: etiology and pathogenesis. Arch GynecolObstet, 2006, 274(4):187-97.
3Homburg R. Polycystic ovary syndrome. Best Pract Res ClinObstet Gynaecol, 2008, 22(2):261-74.
4Catteau-Jonard S,Jamin SP, Leclerc A, et al. Anti-Mullerianhormone, its receptor, FSH receptor, and androgen receptorgenes are overexpressed by granulosa cells from stimulatedfollicles in women with polycystic ovary syndrome. J ClinEndocrinol Metab, 2008,93(11):4456-61.
5Zurvarra FM, Salvetti NR, Mason JI, et al. Disruption in theexpression and immunolocalisation of steroid receptors andsteroidogenic enzymes in letrozole-induced polycystic ova-ries in rat. Reprod Fertil Dev, 2009,21(7):827-39.
6Yuan W, Giudice LC. Programmed cell death in human ovaryis a function of follicle and corpus luteum status. J ClinEndocrinol Metab, 1997, 82(9):3148-55.
7Chamberlain NL, Driver ED, Miesfeld RL. The length andlocation of CAG trinucleotide repeats in the androgen recep-tor N-terminal domain affect transactivation fonction. NucleicAcids Res, 1994,22(15):3181-6.
8Schiiring AN, Welp A, Gromoll J. Role of the CAG repeatpolymorphism of the androgen receptor gene in polycysticovary syndrome (PCOS). Exp Clin Endocrinol Diabetes,2012,120(2):73-9.
9Xia Y,Che Y, Zhang X,et al. Polymorphic CAG repeat in theandrogen receptor gene in polycystic ovary syndromepatients. Mol Med Reprod, 2012, 5(5):1330-4.
10Ellis JA, Stebbing M,Harrap SB. Polymorphism of theandrogen receptor gene is associated with male pattern baldness.J Invest Dermatol, 2001,116(3):452-5.