2型糖尿病大鼠胰腺中Homer的表达及意义

Homer expression in the pancreases of type 2 Diabetes mellitus rats

目的探讨Homer与胰岛素分泌及2型糖尿病发病机制的相关意义。方法建立2型糖尿病大鼠模型,检测血糖和血胰岛素值。取胰腺组织,采用反转录PCR、免疫组化和免疫荧光方法检测Homer(Homer1c、Homer2、Homer3)和胰岛素的表达。结果大鼠胰腺组织可见Homer表达,糖尿病大鼠胰腺组织胰岛素表达明显减少(P<0.01),而Homer1c、Homer2和Homer3均表达增加(P<0.05)。结论 Homer基因在大鼠胰腺组织亦有表达,Homer1c、Homer2和Homer3可能对胰岛素分泌起负性调控作用。

Objective To study the expression and correlation of Homer and insulin in rats with type 2 Diabetes mellitus. Methods Twenty-two Diabetes mellitus rats and 15 normal rats were sacrificed, the pancreases of rats were collected to detect Homer （Homeric, Homer2 and Homer3） and insulin by PCR, immunohistochemistry and double immunofluorescent labeling methods. Results Homerl, Homer2 and Homer3 were positive in the pancrea- ses. In rats with Diabetes mellitus, leverages of insulin was much lower, but expressions of Homerl c, Homer2 and Homer3 protein was much higher. Conclusions The result shows that there is a significant change in the expression of Homerl c, Homer2, Homer3 and insulin between two groups. Homer may play an important role in insulin secre- tion, Homerl c, Homer2, Homer3 may act as inhibitors.