PVA水凝胶、海藻酸钠凝胶与羊椎骨支架粘合植入动物体内观察

Biocompatibility and Degradation of Polyvinyl Alcohol Gel and Sodium Alginate Geln in vivo

目的:研究聚乙烯醇水凝胶、海藻酸钠凝胶在生物体内生物相容性及降解速率,为三维快速打印技术构建组织工程骨的研究奠定基础。方法:实验样本分3组,对照组为单纯羊椎骨支架材料,聚乙烯醇水凝胶-羊椎骨支架组为实验1组,海藻酸钠凝胶-羊椎骨支架组为实验2组,分别植入裸鼠体内后术后4、8周后处死,苏木精-伊红染色后观察。结果:术后4周标本可见两实验组和对照组材料皮下可见大量纤维结缔组织及表皮毛细血管,植入材料周围发现有结缔组织增生并有炎细胞浸润,对照组支架材料边缘呈毛刺状,实验两组胶体吸收不明显,支架材料周围有疏松的纤维囊壁形成;术后8周标本可见对照组支架边缘可见毛刺状表现,炎细胞浸润;实验1组表皮下炎细胞明显增多,毛细血管未见明显扩张,支架材料周围未见破骨细胞粘附;实验2组和对照组材料周围组织炎症反应减轻,少量中性粒细胞和浆细胞浸润;实验2组可见破骨细胞粘附,部分凝胶胶体降解,支架骨材料边缘呈毛刺状。结论:对照组和海藻酸钠凝胶组支架实验材料可短期开始吸收;聚乙烯醇水凝胶组短期无吸收。

Objective： To lay foundation for the study of 3D printing technology to construct tissue engineered bone through investigating in vivo biocompatibility and degradation rate of polyvinyl alcohol gel and sodium alginate gel. Methods： The experimental samples were divided into three groups, the sheep vertebrae scaffold materials as control group, polyvinyl alcohol gel-- sheep vertebrae scaffold as experimental group 1, sodium alginate gel-- sheep verte- brae scaffold as experimental group 2. The three kinds of materials were implanted into nude mice, which was killed in the first four weeks and eight weeks after surgery and then observed the HE dying. Results： Specimens showed a large number of fibrous connective tissue and skin blood capillary in two experimental groups and the control ~roup materials 4 weeks after the surgery. And connective tissue hyperplasia and inflammatory cdlls infiltration were found around the implant materials. The edge of control group was shaped like a burr. The colloidal absorption was not obvious in the two experimental groups and loose fibre capsule walls were formed around the scaffold materials. Eight weeks after surgery, specimens showed burr edges and inflammatory cells infiltrated in the scaffold materials group. Skin inflammatory cells obviously increased, no obvious dilation of capillaries and osteoelast adhere to scaf- fold materials in experimental group 1. The experimental group 2 and control group materials surrounding tissue in- flammation reaction decreased, a small amount of neutrophils and plasma cell infiltrated. Experimental group 2 showed that osteoclast adhesion and a small proportion of the gel colloidal degraded. And there was a burr edge in scaffold bone materials. Conclusion： Short--term absorption was seen in the control group and sodium alginate gel group but not in the polyvinyl alcohol gel group.