应用光谱测量牙周病炎性指标

Measurement of Periodontal Inflammation Index with Spectroscopy

目的:应用光谱测量局部组织的水肿和血流动力学变化,评价光学光谱在不同人群中对牙周组织多种炎性指标(组织的氧合作用、组织的总血红蛋白量、脱氧血红蛋白、含氧血红蛋白和组织水肿)的评估能力。方法:使用手提式的近红外光谱测量仪分别从健康组织区域(n=54)、牙龈炎区域(n=50)、牙周炎区域(n=26)获取光谱并对其进行处理及评估。使用非参数的散射损失函数的改良比尔-兰伯特分解方式来研究每一种炎性组分在整体光谱中的相对分布。结果:光学光谱成功地用于评估牙周组织中复杂的炎症环境。数字表明牙周炎组织的氧合作用与牙龈炎区域及健康组织区相比明显降低(P<0.05)。这主要是由于在牙周炎区域的脱氧血红蛋白含量比牙龈炎区域(P=0.05)及健康组织区(P<0.01)明显增加所造成的。组织的含水量没有统计学差异,但是当牙周炎区域与牙龈炎区域及健康组织区域相比时,水指数相关的组织电解质水平及温度仍有统计学差异(P<0.03)。结论:本项研究进一步证实光学光谱能够在人类牙周组织中直接测量炎性指标。近红外线可见光谱可作为一种简单的、低耗的、无创伤、椅旁的、定位精确的诊断工具用于牙周病的诊断。

Objective： To further evaluate the ability of optical spectroscopy in the different individual groups to de- termine simultaneously multiple inflammatory indices in periodontal tissues in vivo. Methods： Spectra were ob- tained, processed and evaluated from healthy（n= 54）, gingivitis（n= 50） and periodontitis（n= 26） sites using a portable optical, near infrared spectrometer. A modified Beer--Lambert unmixing model that incorporates a non- parametric scattering loss function was still used by our team of international collaboration to determine the relative contribution of each inflammatory component to the overall spectrum. Results; Optical spectroscopy was successful- ly to further regenerate complex inflammatory profiles of periodontal tissues. Tissue oxygenation at periodontitis sites was significantly decreased （P〈0.05） compared to sites with gingivitis and healthy controls. This was largely the result of an increase in deoxyhemoglobin in the periodontitis sites compared with healthy （P〈0.01） and gingivi- tis （p = 0.05）sites. Tissue water content per se showed no significant difference between the sites, hut a water index associated with tissue electrolyte levels and temperature differed significantly between periodontitis sites and both healthy and gingivitis sites（P〈0.03）. Conclusion： Optical spectroscopy can simultaneously determine multiple in flammatory indices directly in the periodontal tissues in the individual group of a whole world. Visible, near--infra red spectroscopy has the potential to be developed into a simple, reagent--free, a noninvasive, chair side, site--spe cific, diagnostic and prognostic test for periodontitis.