多发性骨髓瘤细胞B7-H3分子的表达与预后和骨质破坏的关系

Relation of B7-H3 Molecule Expression in Multiple Myeloma with Poor Prognosis and Bone Destruction

本研究探讨多发性骨髓瘤细胞株及多发性骨髓瘤患者CD138+骨髓瘤细胞中B7-H3的表达水平及其临床意义。应用流式细胞术及RT-PCR法检测3种骨髓瘤细胞株(RPMI8226、U266和H929)表面B7-H3的表达水平及45例(46例次)多发性骨髓瘤患者CD138+细胞中B7-H3的表达水平,分析其与临床预后及生存时间相关性。结果表明:①B7-H3在骨髓瘤细胞株RPMI8226、U266中高表达,阳性率分别为(92.30±1.1)%和(79.03±1.2)%,在H929细胞中未见明显表达,约占(4.26±0.2)%;RT-PCR检测到RPMI8226及U266细胞株B7-H3 mRNA产物,在H929细胞株未检测到其产物。②外源IL-6刺激细胞株未见B7-H3分子的上调。③在多发性骨髓瘤患者中,初诊者B7-H3阳性率为(48.58±33.593)%,缓解者为(22.16±18.853)%,复发者为(57.65±28.296)%,初诊与缓解、缓解与复发者的B7-H3阳性率差异有显著统计学意义(P=0.023,P=0.004)。④B7-H3高表达较低表达的患者具有更多的骨质破坏(P=0.027),血清钙离子水平显著升高(2.3144±0.44619 vs 2.0948±0.2504;P=0.046)。结论:B7-H3的表达可能与多发性骨髓瘤的预后呈负相关,与骨髓骨质破坏程度呈正相关。

This study was purposed to investigate the B7-H3 expression in multiple myeloma cell lines and CD138 cells of patients with multiple myeloma, and explore its clinical significance. Three myeloma cell lines （RPMI8226, U266 and H929） were used. Forty-five patients with multiple myeloma were enrolled in the study. The expression of B7-H3 was detected by flow cytometry and RT-PCR. The relationship between B7-H3 and clinical prognostic factor was analyzed. The results showed that （ 1 ） In myeloma cell lines, high expression of B7-H3 was seen in RPMI8226（92.30 ± 1.1 ） % and U266 （79.03 ± 1.2） % but not in H929 cell line （4.26± 0.2） %. （2） Exogenous IL-6 had no effect on upregulation of B7-H3 in myeloma cell lines. （3） In multiple myeloma patients, the proportions of B7-H3 positive cells in newly diagnosed, remission and relapsed patients were （48.58 ±33. 593 ） %, （ 22.16 ± 18.853 ） %, and （ 57.65 ± 28. 296） %, respectively. The difference between the newly diagnosed and remission patients, and remission and relapsed patients was significant （ P = 0. 023, P = 0. 004 ）. （4） High B7-H3 expression was correlated with high numbers of bone destruction and high levels of serum calcium （ P = 0. 027, P = 0. 046, respectively）. It is concluded that the relation of B7-H3 molecule expression with prognosis of multiple myeloma may be negative, but with degree of bone destuction is positive, thus the high expression of B7-H3 may correlated with disease progression and bone destruction of patients with multiple myeloma.