初发免疫性血小板减少症患者外周血中维生素D及其受体mRNA的表达水平和意义

Expression and Significance of Vitamin D and Its Receptor mRNA in the Peripheral Blood of Initial Immune Thrombocytopenic Patients

为了探讨维生素D(VD)对初发免疫性血小板减少症(ITP)发病与治疗的作用,采用酶联免疫吸附试验(ELISA)检测45例初发ITP患者和30例健康体检者外周血中25羟基维生素D3[25(OH)D3]和1,25-二羟基D3[1,25(OH)2D3]的浓度,利用逆转录一聚合酶链反应(RT-PCR)法检测患者外周血VD受体(VDR)mRNA表达水平。研究结果表明,ITP患者外周血中25(OH)D3(10.6±7.7 ng/ml)和1,25(OH)2D3(69.9±29.0 pg/ml)的浓度明显低于健康对照组(13.7±9.1 ng/ml,87.3±19.9 pg/ml)(P<0.05),而VDR mRNA的表达(1.588±0.127)明显高于健康对照组(1.055±0.734)(P<0.05)。结论:VD水平及其受体的表达异常可能参与了ITP的发病,VD及类似物可用于ITP的治疗。

To investigate the effect of vitamin D in pathogenesis and clinical treatment of patients with immune thrombocytopenic （ITP）, ELISA was used to detect the level of 25-hydroxylate vitamin O3 [ 25 （OH）D3 ] and 1,25-di- hydroxyvitamin D3 [ 1,25 （OH） 2D3 ] in peripheral blood from 45 ITP patients and 30 normal controls. Vitamin D receptor（VDR） mRNA expression was detected by RT-PCR. The results showed that the levels of 25 （OH） D3 （ 10.6 ±7.7 ng/rrfl） and 1,25 （OH） 2D3 （69.9 ± 29.0 pg/rrd） in peripheral blood of the initial ITP patients were lower than those in peripheral blood of normal controls（ 13.7 ±9.1 ng/ml, 87.3±19.9 pg/ml） （P 〈0.05）. The expression of VDR gene obviously increased in peripheral blood of initial ITP patients（ 1. 588 ±0. 127 ）, as compared with that in peripheral blood of normal controls （ 1. 055± 0.734 ） （P 〈 0.05 ）. It is concluded that vitamin D level and its receptor expression may play an important role in ITP, and vitamin D and its similarities may be a new agent to treat patients with ITP.