汉族人群慢性牙周炎患者IL-13和IL-4基因多态性及连锁不平衡研究

Gene polymorphism and linkage disequilibrium of IL-13 and IL-4 in Chinese Han population with chronic periodontitis

目的研究汉族人群慢性牙周炎患者白介素13(IL-13)和IL-4基因多态性分布情况及其与慢性牙周炎发病的可能关系。方法采用基质辅助激光解吸离子化-飞行时间质谱法(MALDI-TOF-MS)对慢性牙周炎组(n=278)和健康对照组(n=324)IL-13基因中rs2070874和rs1800925及IL-4基因中rs2243248的单核苷酸多态性(SNP)进行检测,计算等位基因频率和基因型分布,并进行连锁不平衡及单倍型分析。结果慢性牙周炎组与健康对照组rs1800925的TT基因型和T等位基因与rs2243248的GG基因型和G等位基因频率的差异有统计学意义(P<0.05);两组间rs2070874 T/rs1800925 T/rs2243248 G单倍型及另外两种单倍型rs2070874 T/rs1800925 C/rs2243248 G与rs2070874 T/rs1800925 T/rs2243248 T的分布差异也有统计学意义(P<0.05)。结论 IL-13基因rs1800925和IL-4基因rs2243248的SNP可能与中国汉族人群慢性牙周炎的发病易感性相关。

Objective To investigate the gene polymorphism of interleukin （IL） -13 and IL-4 in Chinese Han population with chronic periodontitis, and explore its potential relationship with chronic periodontitis. Methods Matrix-assisted laser desorption ionization time of flight mass spectrometer （MALDI-TOF-MS） was employed to detect the single nucleotide polymorphism （SNP） of rs2070874 and rs1800925 in IL-13 and rs2243248 in IL-14 in chronic periodontitis group （n = 278） and healthy control group （n = 324）, the allele frequency and genotype distribution were calculated, and linkage disequilibrium and haplotype analysis was conducted. Results There were significant differences in the allelic frequency of rs1800925 TT genotype and T allele, as well as that of rs2243248 GG genotype and G allele between chronic periodontitis group and healthy control group （P 〈 0.05）. There were also significant differences in the distribution of rs2070874 T/rs1800925 T/rs2243248 G, rs2070874 T/rs1800925 C/rs2243248 G and rs2070874 T/rs1800925 T/rs2243248 T between two groups （P 〈 0.05）. Conclusion SNP of rs1800925 in IL-13 gene and rs2243248 in IL-4 gene may be correlated with the susceptibility to chronic periodontitis in Chinese Han population.