摘要
目的:探讨下腔静脉损伤后基质金属蛋白酶(MMP-2,MMP-9)及转化生长因子β1(TGF-β1)的变化规律及粒细胞集落刺激因子(G-CSF)干预的影响。方法:48只家兔制作下腔静脉球囊损伤模型后随机分为实验组(术后应用G-CSF)和模型组(术后应用生理盐水),两组分别于术后3,7,14,28 d取血管损伤段(n=6),应用免疫组化染色检测MMP-2,MMP-9,TGF-β1的表达。结果:在未损伤的家兔下腔静脉中仅有少量的MMP-2和MMP-9表达,而TGF-β1几乎无表达。下腔静脉损伤后3种因子表达均明显升高,并于第7天达高峰,随后回落;G-CSF对3种因子表达的升高过程(3,7 d)无明显干预作用(与对照组比较,均P>0.05),但能明显加速各因子水平的降低过程(14,28 d)(与对照组比较,均P<0.05)。结论:下腔静脉内膜损伤后存在重塑相关因子表达的短期升高,G-CSF对其升高过程无干预作用,但能加速其恢复,该作用有利于抑制血管平滑肌的过度增生与加速再内皮化进程。
Objective: To investigate the alteration patterns of matrix metalloproteinases (MMP-2, MMP-9) and transforming growth factor β1 (TGF-β1) in inferior vena cava (IVC) after injury and the effects exerted by granulocyte colony-stimulating factor (G-CSF). Methods: Forty-eight rabbits were randomly divided into experimental group (treated with G-CSF after operation) and control group (treated with normal saline) after IVC balloon iniury was performed. The injured vascular segments (n=6) in both groups of rabbits were excised on postoperative day (POD) 3, 7, 14, and 28 respectively, for immunochemical staining ofMMP-2, MMP-9 and TGF-β1. Results: In unin)ured rabbit IVC, there was a small amount of MMP-2 and MMP-9 expression, and nearly no TGF-β1 expression. After injury, the expressions of all the three factors in WC were significantly increased, and all of them reached their maximal degrees on POD 7 and then declined. G-CSF treatment showed no effect on the expression of the three factors during the course of their increase (3 and 7 d) (both P〉0.05 vs. control group), but markedly accelerated the decreasing tendency of their levels (14 and 28 d) (both P〈0.05 vs. control group) Conclusion: q-here are short-term increased expressions of remodeling-associated factors in IVC after intimal iniury. G-CSF cannot inhibit the increase of these factors but can accelerate their decrease, and may be favorable for the inhibition of excessive proliferation of the vascular smooth muscle cells and acceleration of the reendothelialization process.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2013年第6期721-725,共5页
China Journal of General Surgery
基金
河南省教育厅科技攻关计划资助项目(2011A320025)
关键词
缩窄
病理性
腔静脉
下
巨噬细胞集落刺激因子
基质金属蛋白酶类
转化生长因子β1
Constriction, Pathologic
Vena Cava, Inferior
Macrophage Colony-Stimulating Factor
Matrix Metalloproteinases
Transforming Growth Factor β1