腹主动脉瘤组织中SM22α与骨桥蛋白以及细胞骨架蛋白表达的改变

Expression alterations of SM22α,osteopontin and cytoskeletal proteins in abdominal aortic aneurysm tissue

目的:探讨SM22α与骨桥蛋白(OPN)以及细胞骨架蛋白α-SMA,β-tubulin,desmin在腹主动脉瘤(AAA)组织中的表达改变。方法:采集正常人腹主动脉壁和AAA动脉壁组织,分别采用real-time PCR与免疫组织化学法检测两种组织中SM22α,OPN,α-SMA,β-tubulin和desmin的mRNA与蛋白表达水平。结果:与正常腹主动脉壁组织比较,AAA组织中的SM22α,α-SMA,β-tubulin和desmin的mRNA与蛋白水平均明显降低,且SM22α表达降低最为明显,而OPN的mRNA与蛋白的表达明显升高(均P<0.05)。结论:在AAA组织中,SM22α与细胞骨架蛋白表达降低,OPN升高,这些改变与AAA的发生可能有密切关系。

Objective： To observe the expression alterations of SM22α, osteopontin （OPN） and cytoskeletal proteins including α-SMA, β-tubulin and desmin in human abdominal aortic aneurysm （AAA） tissues. Methods： The tissues from normal abdominal aortic wall and AAA wall were harvested, and then mRNA and protein expression levels of SM22α, OPN, α-SMA, β-tubulin and desmin in the two types of tissues were determined by real-time PCR assay and immunohistochemical staining, respectively. Results： Compared with normal abdominal aortic tissue, in AAA tissue, both mRNA and protein expressions of SM22α, α-SMA, α-tubulin and desmin were significantly decreased, especially that of SM22α, whereas the mRNA and protein expression levels of OPN were significantly increased （all P〈0.05）.Conclusion： There is decreased expression of SM22α and cytoskeletal protein level, and increased OPN level in AAA tissue, which may be closely associated with the occurrence of AAA.