山羊关节炎脑炎病超微结构的观察

Observation on Ultrastructure of Caprine Arthritis Encephalitis (CAE)

采用关节炎脑炎 (CAE)标准抗原和血清检测萨能奶山羊、繁殖羔羊、杂交奶山羊、地方 (黑、白、麻 )山羊的血清 550份 ,检出 CAE阳性血清 30份 ,可疑 5份。剖解 CAE阳性羊 10只 (其中奶山羊 8只 ,地方山羊 2只 ) ,同时取肝、脾、肺肾、淋巴结、关节膜、大脑、小脑做超微切片。其结果 :(1)临床症状表现为食欲不振 ,被毛粗乱 ,消瘦拉稀 ,跛行或瘫痪 ,精神不振 ;(2 )剖解变化 :脑膜充血、出血 ,关节膜增厚、质硬 ,关节囊内有多量混浊或淡红色液体。各肺小叶表面都有大小不等的坏死灶 ,质地较硬。胆囊增大。心包膜都有出血点。肠系膜淋巴结轻微肿大 ;(3)超微结构变化 :大脑、小脑分子层的星形细胞、水平细胞以及多形细胞层的细胞和大小锥体细胞、蒲金野氏细胞胞浆中的线粒体肿胀 ,并变成圆形或不规则形 ,线粒体膜不清或消失 ,整个线粒体变性、坏死 ,溶解、消失 ,最后形成空腔。核膜结构清楚或不清 ,核孔增大 ,染色质边移或成团块状。核内基质减少 ,核仁消失 ,电子密度中度。肺、肾、肝脏的上皮细胞内线粒体肿胀、变性、坏死 ,胞核内染色质疏松 ,也可见团块状 ,电子密度较低或中度 ,核孔增大。淋巴结和脾脏中的淋巴细胞内线粒体变性、坏死、消失 ,然后变成空腔 ,但核膜清楚 ,染色质均匀 ,电子密度中度。网状细?

The standard antigen and serum of the OPP and CAE were used to examine 550 sera of saanen milk goats, dark, white, flaxen goats. Result indicated clearly:(1)There were 30 CAE positive reaction and 5 suspicious reaction in examination total goats 10 saanen milk goats and local goats of CAE positive reaction had been dissected and the liver, cerebrum, stomach, intestine had been taken to do section of the paraffin wax. (2)Clinical symptoms: the goats of the disease appeared to has a jaded appetite to rough quilt hair, to become thin and to has diarrhea lamed, paralysis, mental no well. (3)Analyse change: mater hyperemia, link area of brain and spinal cord had not haemorrhage. There were must turbid and pale red fluids in the joint capsule. There were necrosis focuses on lung surface with different size and qulaity of a lung changed and gallbladder had been increased big. (4) Ultrastructure change: the cerebellum and cerebrum:mitochondria in the stellate cells of the cerebellum and cerebrum, level cells of the molecular layer, cells of the multiform layer and small pyramidal cells, large pyramidal cells and purkinje's cells cytoplasm happened swelling to form circel and irregular shape. Mitochondria membrane had been observed to be not clear or dissappear. A number of mitochondria occurred degeneration, necrosis disolving, disappears to form cavity. Nuclear envelope of cells was clear or not clear. Nuclear envelope hole of cells were enlarged, and chromatin were moved at verge of nuclear envelope or formed circle and lung shape. Nucleoplasms were reduced, nucleolus's were disappear and electron density were medium. Mitochondria in the epithelial cells of lung, kidney, liver and lymphocytes, macrophages, reticular cells of lymph node spleen occurred swelling, degeneration, necrosis. Chromatin were be formed circle, and lumpshape and electron density were medium. Nuclear envelope hole of cells were enlarged.