Ang-(1-7)对高糖诱导的小鼠胰岛素分泌细胞NIT-1氧化应激的影响

Effect of angiotensin-(1-7) on oxidative stress induced by high glucose of NIT-1 cells

目的观察血管紧张素-(1-7)[Ang-(1-7)]对高糖诱导的小鼠胰岛素分泌细胞株NIT-1氧化应激的影响。方法将对数生长期的NIT-1细胞随机分成A、B、C、D、E组,A组不处理,B、C、D、E组分别用终浓度为10-5mol/L的Ang-(1-7)、35 mmol/L葡萄糖、10-5mol/L的Ang-(1-7)+35 mmol/L葡萄糖、10-5mol/L的Mas受体拮抗剂A-779+10-5mol/L的Ang-(1-7)+35 mmol/L葡萄糖干预72 h。用DCFH-HA荧光染色法检测活性氧簇(ROS),硫代巴比妥酸法测定丙二醛(MDA)。结果 A、B、C、D、E组ROS分别为24.25±1.85、25.31±3.60、33.14±3.79、21.47±1.66、31.71±3.88,MDA分别为(17.99±0.40)、(17.29±1.97)、(30.47±2.61)、(21.57±1.32)、(29.62±3.23)μmol/g;C、E组与A、B、D组比较,P均<0.05;C、E组比较,P均>0.05;A、B、D组比较,P均>0.05。结论Ang-(1-7)通过Mas受体抑制高糖诱导的NIT-1细胞氧化应激。

Objective To observe the effect of angiotensin-（1-7） [ Ang-（1-7） ] on oxidative stress induced by high glucose in mouse insulin-secreting cell line NIT-1. Methods NIT-1 cells in logarithmic phase were divided into five groups： group A with no treatment; the cells in group B, C, D and E were treated with 10^-5 mol/L Ang-（ 1-7 ） , 35 mmol/ L glucose, 10^-5 mol/L Ang-（1-7） ＋ 35 mmol/L glucose, receptor inhibitor （A-779, 10^-5 mol/L ） ＋ 10^-5 mol/L Ang-（ 1- 7） ＋ 35 mmol/L glucose respectively. Every group was treated for 72 hours. Reactive oxygen species （ROS） was detected by DCFH-DA fluorescent staining assay. Malondialdehyde （MDA） was measured by TBA method. Results Concentrations of ROS in group A, B, C, D and E were 24.25 ± 1.85, 25.31 ±3.60, 33.14 ±3.79, 21.47 ±1.66 and 31.71 ±3. 88 respectively. Concentrations of MDA in group A, B, C, D and E were （17.99 ±0.40） , （17.29 ±1.97）, （30.47 ±2.61 ）, （21.57 ±1.32） and （29.62 ±3.23 ） μmol/g respectively. Group C and E were statistically different from group A, B and D （ all P 〈 0.05 ）. There was no statistical difference between group C and E （ P 〉 0.05 ） , meanwhile, there was no statistical difference among group A, B and D （all P 〉 0.05 ）. Conclusion Ang-（ 1-7 ） treatment can block the effect of high glucose on oxidative stress in NIT-1 cells via Mas receptor.