拮抗细菌HD-86发酵液稳定性及对相似穿孔线虫的活性

Stability of the fermentation supernatant from the antagonistic bacterium HD-86 and its activity against Radopholus similis

利用PCR特异性引物检测了一株对相似穿孔线虫具有高拮抗活性的Burkholderia cepacia菌株HD-86的毒性,结果未检测到该菌株中与人体致病相关的洋葱伯克霍尔德氏菌致病因子(BCESM)毒力基因,从而验证了其对人类和环境的安全性。菌株HD-86发酵上清液对相似穿孔线虫拮抗活性稳定性的研究结果表明,经80℃高温处理60min后其拮抗活性仍然极高,在pH为8～10的范围内随着碱性增强其拮抗性效果越好,紫外线处理对其拮抗活性没有影响。

By using PCR specific primers, the potential virulence of Burkholderia cepacia strain HD-86 to human body, which was highly antagonistic against Radopholus similis, was detected. The results indicated that the viru- lence gene of B. cepacia epidemic strain marker （BCESM） did not exist. Thus the strain was deemed safe for hu- man and environment. The results on the stability of fermentation supernatant from the strain HD-86 against R. similes showed that the antagonistic activity remained high at 80℃ for 60 rain, and the antagonistic activity was higher with increasing basicity within the scope of pHS-10. The antagonistic activity was not affected by the ultra- violet ray treatment.