危害红掌的穿孔线虫形态和分子鉴定

Morphological and molecular identification of the burrowing nematode Radopholus similis from Anthurium andraeanum

对一批引进后隔离培养中患病的红掌种苗,进行了根系线虫检测,通过病症分析、线虫形态测量、rDNA-ITS区序列分析等鉴定,结果表明:检测的线虫形态和香蕉穿孔线虫基本一致,rDNA-ITS区测序结果序列一致性为98%～100%。本报道再次证明香蕉穿孔线虫在红掌上的分布和危害,应该对种苗的跨区引进和种植过程进行严格的检疫。

Diagnosis of root parasitic nematodes was carried out on a group of imported and isolatea growing An- thurium andraeanum seedlings with nematode-suspected disease symptoms by symptom diagnosis, morphological measure and rDNA-ITS sequence analysis. The isolated nematode was identified to be burrowing nematode Radopholus similis. The results revealed that the burrowing nematode could exist and damage on A. andraeanum. Take into account its wide host range and gigantic harmfulness, a strict inspection and quarantine should be ap- plied in the importation and cultivation of A. andraeanurn.