紫外分光光度法测定复方氢醌脂质体含量和包封率

Determination of Co-Hydroquinone-Liposome by ultraviolet spectrophotometry

目的建立可用于检测复方氢醌脂质体浓度及包封率的紫外分光光度法。方法采用紫外分光光度法,在294、340nm分别检测氢醌和维甲酸的紫外吸收。结果氢醌在30～240μg/mL范围内线性关系良好(r2=0.99,n=5),方法回收率为96.6%,相对标准偏差为4.7%;维甲酸在5～40μg/mL范围内线性关系良好(r2=0.99,n=5),方法回收率为93.3%,相对标准偏差为4.9%。结论该测定方法简便快速,可用于复方氢醌脂质体浓度和包封率体外检测。

Objective To establish a method for determining the content and encapsulation efficiency of co-hy- droquinone-liposome. Methods The method of ultraviolet spectrophotometry was used,and the detection wavelength was 294 and 340 nm respectively. Results The detection linear ranges of hydroquinone was 30--240μg/mL（r2 0.99, n= 5） ,and average recovery of hydroquinone was 96.6 %±4.7% The detection linear ranges of vitamin A acid was 5--40 Ixg/mL（r2 = 0.99, n = 5）. The average recovery of vitamin A acid was 93.3%fro ±4.9%. Conclusion The method was simple and convenient,and could meet the need for detection Co-Hydroquinone-liposome content and en- capsulation efficiency. [Key words] liposome; hydroquinone; vitamin A acid; encapsulation efficiency