稻瘟病菌致病突变株的分离

Isolation of Pathogenic Mutants of Magnaporthe Grisea

利用 4个分属宗亲群 4,7,1 7和 44的菌株连续接种在C0 39的单基因近等基因系和基因累加系上 ,以获得突变抗病基因新菌株。经 1 3轮接种循环 ,共分离到 9个新菌株。其中 8个针对C1 0 1LAC(Pi- 1 )突变 ,另 1个针对C1 0 1PKT (Pi- 4a)和F1 2 8(Pi-ta2 )的双位点突变。 4个原始菌株 ,P0 6 - 6 ,92 48- 6 ,92 39- 4和 1 0 1 - 7-2的突变频率分别为 :0 ,2 82× 1 0 -5,2 0 5× 1 0 -5和 9 0 9× 1 0 -5。菌株针对不同抗病基因的突变频率不同 ,由Av- 1变到Av - 1 + 和由Avir - 4a(t)突变到Av - 4a+ 的频率分别为 7 5 1× 1 0 -5和 6 1 4× 1 0 -5。没有针对Pi- 2突变体出现。

To isolate pathogenic mutants of Magnaporthe grisea , 4 parental strains belonging to lineages 4, 7, 17 and 44 were inoculated onto the C039 near isogenic lines successively.Nine mutants were obtained after 13 inoculation cycles Among the mutants, 8 mutated to C101LAC (Pi-1) and 1 to C101PKT (Pi-4) and F128 (Pi-ta 2). The four parental strains, P06-6, 9248-6, 9239-4 and 101-7-2-1-1 mutated with frequencies of 0, 2 82×10 -5 , 2 05×10 -5 and 9 09×10 -5 , respectively. The mutation frequencies were different for different resistance genes of rice.The frequencies were 7 51×10 -5 and 6 14×10 -5 for Pi-1 and Pi-4.No mutants appeared on C101A51 (Pi-2).