香菇单孢杂交及杂交菌株分子鉴定

Single-spore Crossing of Lentinula edodes and Identification of Hybrid Strains Using Inter-Simple Sequence Repeat （ISSR） Markers

采用单孢杂交育种技术，以香菇（Lentinula edodes）栽培品种“大山18”和云南野生香菇“11．1”为亲本，选育香菇优良新品种。通过单孢分离和配对杂交，共获得84个杂交菌株。用ISSR分子标记对杂交菌株与亲本条带进行鉴定，有45个菌株包含了两亲本的所有遗传条带，最终证明了这45个菌株是真正的杂合子。本研究表明了利用ISSR标记可以有效地对早期菌丝体阶段的香菇杂交菌株的真实性进行鉴定。

A Lentinula edodes cultivar, ＂Dashan 18＂, and strain ＂11-1＂, collected from the wild in Yunnan Province, were selected as parental strains in a single-spore cross-breeding experiment that generated 84 putative hybrids. ISSR methodology demonstrated specific bands from both parental strains in 45 hybrid strains, confirming the latter were true hybrids, and indicating that ISSR markers can be used effectively to identify L. edodes hybrid strains growing in the vegetative phase.